Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 3094856..3095481 Replicon chromosome
Accession NZ_CP009050
Organism Escherichia coli NCCP15648

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag A610_RS16615 Protein ID WP_000911329.1
Coordinates 3094856..3095254 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag A610_RS16620 Protein ID WP_000450524.1
Coordinates 3095254..3095481 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A610_RS16595 3090734..3090934 + 201 WP_000383836.1 YpfN family protein -
A610_RS16600 3091044..3091742 - 699 WP_000679823.1 esterase -
A610_RS16605 3091816..3093831 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
A610_RS16610 3093846..3094709 - 864 WP_001267498.1 neutral zinc metallopeptidase -
A610_RS16615 3094856..3095254 - 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
A610_RS16620 3095254..3095481 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
A610_RS16625 3095636..3096349 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
A610_RS16630 3096562..3097596 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
A610_RS16635 3097613..3098491 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
A610_RS16640 3098637..3099209 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
A610_RS16645 3099209..3099679 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T48187 WP_000911329.1 NZ_CP009050:c3095254-3094856 [Escherichia coli NCCP15648]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T48187 NZ_CP009050:c3095254-3094856 [Escherichia coli NCCP15648]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT48187 WP_000450524.1 NZ_CP009050:c3095481-3095254 [Escherichia coli NCCP15648]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT48187 NZ_CP009050:c3095481-3095254 [Escherichia coli NCCP15648]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References