Detailed information of TA system TA048131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 10384..10973
• Replicon: plasmid pXCAW58
• Accession: NZ_CP009036
• Organism: Xanthomonas citri pv. citri strain AW15

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: AMD07_RS00140
• Protein ID: WP_015463824.1
• Coordinates: 10641..10973 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: AMD07_RS00135
• Protein ID: WP_011052949.1
• Coordinates: 10384..10641 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMD07_RS00120	5691..6116	+	426	Protein_6	tyrosine-type recombinase/integrase	-
AMD07_RS00125	6161..9121	+	2961	WP_015472939.1	Tn3 family transposase	-
AMD07_RS23210	9288..9592	-	305	Protein_8	Tn3 family transposase	-
AMD07_RS00130	9576..10178	-	603	WP_011052950.1	recombinase family protein	-
AMD07_RS00135	10384..10641	+	258	WP_011052949.1	antitoxin	Antitoxin
AMD07_RS00140	10641..10973	+	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AMD07_RS25410	10996..11247	+	252	WP_125459571.1	hypothetical protein	-
AMD07_RS00145	11244..11456	+	213	WP_011052947.1	hypothetical protein	-
AMD07_RS00150	11446..11982	+	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
AMD07_RS25415	12272..12493	+	222	WP_011052944.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..58317	58317

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T48131 WP_015463824.1 NZ_CP009036:10641-10973 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T48131 NZ_CP009036:10641-10973 [Xanthomonas citri pv. citri]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT48131 WP_011052949.1 NZ_CP009036:10384-10641 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT48131 NZ_CP009036:10384-10641 [Xanthomonas citri pv. citri]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6