Detailed information of TA system TA048069

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 18217..18806
• Replicon: plasmid pXAC33
• Accession: NZ_CP009011
• Organism: Xanthomonas citri pv. citri strain JX4

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: AMD21_RS00110
• Protein ID: WP_015463824.1
• Coordinates: 18217..18549 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: AMD21_RS00115
• Protein ID: WP_011052949.1
• Coordinates: 18549..18806 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMD21_RS24455	16697..16918	-	222	WP_011052944.1	hypothetical protein	-
AMD21_RS00100	17208..17744	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
AMD21_RS00105	17734..17946	-	213	WP_011052947.1	hypothetical protein	-
AMD21_RS24460	17943..18194	-	252	WP_125459571.1	hypothetical protein	-
AMD21_RS00110	18217..18549	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AMD21_RS00115	18549..18806	-	258	WP_011052949.1	antitoxin	Antitoxin
AMD21_RS00120	19012..19614	+	603	WP_011052950.1	recombinase family protein	-
AMD21_RS22405	19598..19902	+	305	Protein_28	Tn3 family transposase	-
AMD21_RS00125	20069..20275	-	207	Protein_29	Tn3 family transposase	-
AMD21_RS22410	20320..20889	-	570	Protein_30	UPF0149 family protein	-
AMD21_RS00130	20908..22122	-	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
AMD21_RS24465	22196..22378	-	183	Protein_32	UPF0149 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33695	33695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T48069 WP_015463824.1 NZ_CP009011:c18549-18217 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T48069 NZ_CP009011:c18549-18217 [Xanthomonas citri pv. citri]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT48069 WP_011052949.1 NZ_CP009011:c18806-18549 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT48069 NZ_CP009011:c18806-18549 [Xanthomonas citri pv. citri]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6