Detailed information of TA system TA048053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 14321..14910
• Replicon: plasmid pXAC33
• Accession: NZ_CP009005
• Organism: Xanthomonas citri pv. citri strain MF20

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: AMD10_RS00090
• Protein ID: WP_015463824.1
• Coordinates: 14321..14653 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: AMD10_RS00095
• Protein ID: WP_011052949.1
• Coordinates: 14653..14910 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMD10_RS24620	12801..13022	-	222	WP_011052944.1	hypothetical protein	-
AMD10_RS00080	13312..13848	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
AMD10_RS00085	13838..14050	-	213	WP_011052947.1	hypothetical protein	-
AMD10_RS24625	14047..14298	-	252	WP_125459571.1	hypothetical protein	-
AMD10_RS00090	14321..14653	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AMD10_RS00095	14653..14910	-	258	WP_011052949.1	antitoxin	Antitoxin
AMD10_RS00100	15116..15718	+	603	WP_011052950.1	recombinase family protein	-
AMD10_RS22535	15702..16006	+	305	Protein_24	Tn3 family transposase	-
AMD10_RS00105	16173..16379	-	207	Protein_25	Tn3 family transposase	-
AMD10_RS22540	16424..16993	-	570	Protein_26	UPF0149 family protein	-
AMD10_RS00110	17012..18226	-	1215	WP_011050083.1	IS4-like element ISXac1 family transposase	-
AMD10_RS24630	18300..18482	-	183	Protein_28	UPF0149 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..26933	26933

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T48053 WP_015463824.1 NZ_CP009005:c14653-14321 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T48053 NZ_CP009005:c14653-14321 [Xanthomonas citri pv. citri]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT48053 WP_011052949.1 NZ_CP009005:c14910-14653 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT48053 NZ_CP009005:c14910-14653 [Xanthomonas citri pv. citri]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6