Detailed information of TA system TA048033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 2549983..2550616
• Replicon: chromosome
• Accession: NZ_CP008998
• Organism: Xanthomonas citri pv. citri strain MN12

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AMD14_RS11565
• Protein ID: WP_015463212.1
• Coordinates: 2549983..2550396 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: M4TWF0
• Locus tag: AMD14_RS11570
• Protein ID: WP_015463213.1
• Coordinates: 2550383..2550616 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AMD14_RS11555	2545675..2547855	+	2181	WP_011051394.1	TonB-dependent siderophore receptor	-
AMD14_RS11560	2548064..2549578	+	1515	WP_015463210.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
AMD14_RS23385	2549594..2549956	-	363	WP_015463211.1	DUF3742 family protein	-
AMD14_RS11565	2549983..2550396	-	414	WP_015463212.1	PIN domain-containing protein	Toxin
AMD14_RS11570	2550383..2550616	-	234	WP_015463213.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AMD14_RS11575	2550874..2551905	+	1032	WP_040107627.1	DUF4917 family protein	-
AMD14_RS11580	2552038..2552436	-	399	WP_099521978.1	hypothetical protein	-
AMD14_RS11585	2552888..2553463	+	576	WP_011051400.1	sigma-70 family RNA polymerase sigma factor	-
AMD14_RS11590	2553444..2554493	+	1050	WP_011051401.1	FecR domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-119)

Antitoxin

• SpoVT-AbrB domain (5-45)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15221.65 Da
• Isoelectric Point: 6.3225

>T48033 WP_015463212.1 NZ_CP008998:c2550396-2549983 [Xanthomonas citri pv. citri]
MAAGKGKVFLDSNVVLYLLSEDAVKADGAEALLQRRPVISVQVLNEVTHVCVRKLKMGWDEVGQFLALVRSFCKIVPLTV
DVHDRARQLAERHQLSFYDACIVAAAAIEGCQTLYSEDMHHGLIIEESLSIRNPFNV

Nucleotide

• Length: 414 bp

>T48033 NZ_CP008998:c2550396-2549983 [Xanthomonas citri pv. citri]
ATGGCCGCGGGTAAGGGCAAAGTGTTTCTCGACAGCAACGTGGTGCTCTACCTGCTGTCGGAGGATGCGGTGAAGGCGGA
CGGCGCGGAGGCATTGCTGCAGCGCAGGCCCGTCATCAGTGTGCAGGTGCTCAATGAAGTGACCCACGTCTGCGTTCGCA
AGCTCAAGATGGGCTGGGACGAAGTCGGGCAGTTCCTTGCGTTGGTGCGATCGTTCTGCAAGATAGTGCCTCTGACGGTC
GATGTTCACGACCGTGCGCGCCAGCTGGCGGAGCGGCACCAATTGTCGTTCTACGACGCGTGCATCGTGGCGGCGGCCGC
CATCGAAGGGTGCCAGACCCTCTACTCCGAAGACATGCACCACGGCCTCATCATCGAAGAGAGCCTGTCGATTCGGAACC
CGTTCAACGTCTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8663.97 Da
• Isoelectric Point: 8.7848

>AT48033 WP_015463213.1 NZ_CP008998:c2550616-2550383 [Xanthomonas citri pv. citri]
MQVAKWGNSLAVRLPSSLVEALELREGDDIEIVVDDPRLFAVRKKPGPEAMLERLRAFRGKLPADFKFNRDEANGRG

Nucleotide

• Length: 234 bp

>AT48033 NZ_CP008998:c2550616-2550383 [Xanthomonas citri pv. citri]
ATGCAAGTCGCGAAGTGGGGTAACAGCCTGGCGGTGCGCCTGCCGTCCAGTCTGGTGGAAGCGCTGGAACTGCGCGAGGG
CGACGACATCGAGATCGTCGTCGATGATCCGCGATTGTTCGCGGTGCGCAAGAAGCCGGGTCCGGAGGCCATGTTGGAGC
GGCTGCGTGCCTTCCGGGGCAAGTTGCCCGCAGATTTCAAGTTCAACCGGGACGAGGCCAATGGCCGCGGGTAA

Structures

Antitoxin

• AlphaFold DB: M4TWF0