Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 51891..52416 Replicon plasmid unnamed
Accession NZ_CP008958
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag EDL933_RS28850 Protein ID WP_001159868.1
Coordinates 52111..52416 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag EDL933_RS28845 Protein ID WP_000813634.1
Coordinates 51891..52109 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EDL933_RS32955 47174..47404 + 231 WP_010891288.1 hypothetical protein -
EDL933_RS28810 47525..48265 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
EDL933_RS28815 48550..49527 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
EDL933_RS28825 49935..50135 - 201 WP_000708307.1 hypothetical protein -
EDL933_RS28830 50132..50752 - 621 WP_001248529.1 hypothetical protein -
EDL933_RS28835 50749..51432 - 684 WP_010891291.1 DNA-binding protein -
EDL933_RS28845 51891..52109 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EDL933_RS28850 52111..52416 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
EDL933_RS28855 52417..53223 + 807 WP_000016989.1 site-specific integrase -
EDL933_RS31890 54485..54823 + 339 WP_071525396.1 RepB family plasmid replication initiator protein -
EDL933_RS28870 55411..56577 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB 1..92076 92076


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T48004 WP_001159868.1 NZ_CP008958:52111-52416 [Escherichia coli O157:H7 str. EDL933]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T48004 NZ_CP008958:52111-52416 [Escherichia coli O157:H7 str. EDL933]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT48004 WP_000813634.1 NZ_CP008958:51891-52109 [Escherichia coli O157:H7 str. EDL933]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT48004 NZ_CP008958:51891-52109 [Escherichia coli O157:H7 str. EDL933]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References