Detailed information of TA system TA048003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 4810..5369
• Replicon: plasmid unnamed
• Accession: NZ_CP008958
• Organism: Escherichia coli O157:H7 str. EDL933

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: EDL933_RS28600
• Protein ID: WP_000421248.1
• Coordinates: 5094..5369 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: EDL933_RS28595
• Protein ID: WP_001178089.1
• Coordinates: 4810..5094 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EDL933_RS28555	1..561	+	561	WP_000840472.1	fertility inhibition protein FinO	-
EDL933_RS28560	694..906	+	213	WP_001302189.1	ANR family transcriptional regulator	-
EDL933_RS28565	1151..1612	+	462	WP_001233853.1	thermonuclease family protein	-
EDL933_RS28570	1658..1867	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
EDL933_RS28575	1905..2243	+	339	WP_000766796.1	hypothetical protein	-
EDL933_RS28580	2483..2737	+	255	WP_000083831.1	replication regulatory protein RepA	-
EDL933_RS28585	2973..3047	+	75	WP_001370046.1	RepA leader peptide Tap	-
EDL933_RS28590	3040..3897	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
EDL933_RS28595	4810..5094	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
EDL933_RS28600	5094..5369	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EDL933_RS28605	5464..5670	+	207	WP_001105064.1	hypothetical protein	-
EDL933_RS29325	5770..5985	-	216	Protein_11	transposase	-
EDL933_RS31850	6029..6595	-	567	Protein_12	IS3-like element IS600 family transposase	-
EDL933_RS28620	6595..7011	-	417	Protein_13	IS91 family transposase	-
EDL933_RS28625	7011..7196	-	186	WP_001302179.1	hypothetical protein	-
EDL933_RS28630	7373..9583	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
EDL933_RS28635	9627..10016	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB	1..92076	92076

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T48003 WP_000421248.1 NZ_CP008958:5094-5369 [Escherichia coli O157:H7 str. EDL933]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T48003 NZ_CP008958:5094-5369 [Escherichia coli O157:H7 str. EDL933]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT48003 WP_001178089.1 NZ_CP008958:4810-5094 [Escherichia coli O157:H7 str. EDL933]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT48003 NZ_CP008958:4810-5094 [Escherichia coli O157:H7 str. EDL933]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8