Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4447076..4447707 Replicon chromosome
Accession NZ_CP008957
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag EDL933_RS23220 Protein ID WP_001259386.1
Coordinates 4447432..4447707 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag EDL933_RS23215 Protein ID WP_000593555.1
Coordinates 4447076..4447435 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EDL933_RS23185 4442188..4442661 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
EDL933_RS23190 4442658..4442939 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
EDL933_RS23195 4443016..4444374 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
EDL933_RS23200 4444367..4445875 + 1509 WP_001301773.1 carbohydrate kinase -
EDL933_RS23205 4445865..4446134 + 270 WP_000933060.1 HPr family phosphocarrier protein -
EDL933_RS23210 4446166..4447026 + 861 WP_001131788.1 class II aldolase -
EDL933_RS23215 4447076..4447435 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EDL933_RS23220 4447432..4447707 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
EDL933_RS23225 4447780..4448904 - 1125 WP_001301659.1 ABC transporter permease -
EDL933_RS23230 4448904..4451639 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
EDL933_RS23235 4451636..4452703 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T47998 WP_001259386.1 NZ_CP008957:c4447707-4447432 [Escherichia coli O157:H7 str. EDL933]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T47998 NZ_CP008957:c4447707-4447432 [Escherichia coli O157:H7 str. EDL933]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT47998 WP_000593555.1 NZ_CP008957:c4447435-4447076 [Escherichia coli O157:H7 str. EDL933]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT47998 NZ_CP008957:c4447435-4447076 [Escherichia coli O157:H7 str. EDL933]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References