Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 4404476..4405276 Replicon chromosome
Accession NZ_CP008957
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name ataT Uniprot ID Q8XED1
Locus tag EDL933_RS22970 Protein ID WP_000342450.1
Coordinates 4404476..4405003 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag EDL933_RS22975 Protein ID WP_001277108.1
Coordinates 4405010..4405276 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EDL933_RS22945 4399551..4400318 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
EDL933_RS22950 4400315..4401592 - 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
EDL933_RS22955 4401589..4402515 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
EDL933_RS22960 4402563..4403672 - 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
EDL933_RS22965 4404096..4404479 + 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
EDL933_RS22970 4404476..4405003 - 528 WP_000342450.1 GNAT family N-acetyltransferase Toxin
EDL933_RS22975 4405010..4405276 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
EDL933_RS22980 4405426..4406529 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
EDL933_RS22985 4406800..4407654 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
EDL933_RS22990 4407899..4408957 - 1059 WP_001042018.1 permease-like cell division protein FtsX -
EDL933_RS22995 4408950..4409618 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19678.61 Da        Isoelectric Point: 6.9489

>T47997 WP_000342450.1 NZ_CP008957:c4405003-4404476 [Escherichia coli O157:H7 str. EDL933]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMNVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T47997 NZ_CP008957:c4405003-4404476 [Escherichia coli O157:H7 str. EDL933]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAACGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT47997 WP_001277108.1 NZ_CP008957:c4405276-4405010 [Escherichia coli O157:H7 str. EDL933]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT47997 NZ_CP008957:c4405276-4405010 [Escherichia coli O157:H7 str. EDL933]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7CHD

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References