Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 546155..546773 Replicon chromosome
Accession NZ_CP008957
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EDL933_RS02640 Protein ID WP_001291435.1
Coordinates 546155..546373 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EDL933_RS02645 Protein ID WP_000344800.1
Coordinates 546399..546773 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EDL933_RS02610 541442..542014 + 573 WP_000779831.1 YbaY family lipoprotein -
EDL933_RS02615 542047..542358 - 312 WP_000409911.1 MGMT family protein -
EDL933_RS02620 542737..543090 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EDL933_RS02625 543132..544682 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EDL933_RS02630 544846..545316 - 471 WP_000136192.1 YlaC family protein -
EDL933_RS02635 545432..545983 - 552 WP_000102553.1 maltose O-acetyltransferase -
EDL933_RS02640 546155..546373 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EDL933_RS02645 546399..546773 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EDL933_RS02650 547319..550468 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
EDL933_RS02655 550491..551684 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T47978 WP_001291435.1 NZ_CP008957:c546373-546155 [Escherichia coli O157:H7 str. EDL933]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T47978 NZ_CP008957:c546373-546155 [Escherichia coli O157:H7 str. EDL933]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT47978 WP_000344800.1 NZ_CP008957:c546773-546399 [Escherichia coli O157:H7 str. EDL933]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT47978 NZ_CP008957:c546773-546399 [Escherichia coli O157:H7 str. EDL933]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References