Detailed information of TA system TA047967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4635665..4636299
• Replicon: chromosome
• Accession: NZ_CP008943
• Organism: Yersinia pseudotuberculosis strain ATCC 6904

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0T9QKF4
• Locus tag: DJ40_RS21020
• Protein ID: WP_025383392.1
• Coordinates: 4635665..4635841 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A380Q6Q6
• Locus tag: DJ40_RS21025
• Protein ID: WP_038401436.1
• Coordinates: 4635892..4636299 (+)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DJ40_RS20975	4630698..4631453	+	756	WP_038401885.1	serine/threonine protein kinase	-
DJ40_RS20980	4631450..4632187	+	738	WP_038401426.1	protein phosphatase 2C domain-containing protein	-
DJ40_RS20985	4632272..4632610	+	339	WP_038401427.1	phage holin, lambda family	-
DJ40_RS20990	4632600..4632995	+	396	WP_038401429.1	M15 family metallopeptidase	-
DJ40_RS20995	4633231..4633698	+	468	WP_038401887.1	lysis protein	-
DJ40_RS21000	4633909..4634091	+	183	WP_038401431.1	hypothetical protein	-
DJ40_RS21005	4634328..4634696	+	369	WP_038401432.1	hypothetical protein	-
DJ40_RS23100	4634727..4635332	+	606	WP_071840332.1	terminase small subunit	-
DJ40_RS21020	4635665..4635841	+	177	WP_025383392.1	type II toxin-antitoxin system HicA family toxin	Toxin
DJ40_RS21025	4635892..4636299	+	408	WP_038401436.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
DJ40_RS21030	4636356..4637960	+	1605	WP_038401438.1	bacteriophage TerL protein	-
DJ40_RS21035	4638058..4638540	-	483	WP_144243032.1	DUF1073 domain-containing protein	-
DJ40_RS21040	4638604..4639245	+	642	WP_200876958.1	hypothetical protein	-
DJ40_RS21045	4639249..4639470	+	222	WP_038401443.1	hypothetical protein	-
DJ40_RS21050	4639463..4641244	+	1782	WP_038401445.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4623642..4642484	18842

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (79-131)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6649.82 Da
• Isoelectric Point: 11.4350

>T47967 WP_025383392.1 NZ_CP008943:4635665-4635841 [Yersinia pseudotuberculosis]
VKQREFQRWLAGQGAEFSNGTNHLKIFLNGKQTIMPRHPGKEIPEPLRKAILKQLGLK

Nucleotide

• Length: 177 bp

>T47967 NZ_CP008943:4635665-4635841 [Yersinia pseudotuberculosis]
GTGAAACAACGCGAGTTCCAGCGTTGGCTAGCGGGGCAAGGAGCTGAATTTTCGAATGGCACTAATCACTTGAAGATTTT
CTTAAACGGCAAGCAAACGATAATGCCAAGACATCCAGGGAAAGAAATACCGGAGCCTCTGAGAAAAGCGATTCTTAAGC
AACTTGGACTTAAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14784.90 Da
• Isoelectric Point: 4.5034

>AT47967 WP_038401436.1 NZ_CP008943:4635892-4636299 [Yersinia pseudotuberculosis]
MRYPIKFEHDETGWCISFPDIPEALTGGATKEEALSLAQDALVTSFDFYFEDQRPVPMPSTDGDEFIEVPASVAAKVLLL
NAMLATGTTPAELARRLGTRPQDVNRIVNLGHTTKIDTIEAALKALGKRLEINVL

Nucleotide

• Length: 408 bp

>AT47967 NZ_CP008943:4635892-4636299 [Yersinia pseudotuberculosis]
ATGCGATATCCAATAAAATTTGAGCATGACGAAACCGGGTGGTGTATATCGTTCCCGGATATACCCGAGGCATTAACTGG
CGGGGCTACAAAGGAAGAAGCGCTATCATTGGCGCAAGACGCCCTTGTAACGTCGTTTGATTTCTATTTTGAAGACCAGC
GCCCGGTACCCATGCCGAGCACAGACGGTGATGAGTTTATCGAGGTGCCTGCAAGCGTGGCCGCCAAAGTGCTATTGCTT
AACGCTATGCTGGCTACAGGAACAACACCGGCAGAGCTTGCCCGCCGCCTAGGTACACGACCGCAAGACGTTAACCGCAT
TGTTAATCTTGGGCACACGACAAAAATCGACACGATAGAAGCGGCGCTTAAGGCGCTAGGTAAGCGTTTAGAAATAAACG
TTCTCTAA

Structures

Toxin

• AlphaFold DB: A0A0T9QKF4

Antitoxin

• AlphaFold DB: A0A380Q6Q6