47950

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3303462..3304060 Replicon chromosome
Accession NZ_CP008934
Organism Geobacillus stearothermophilus 10

Toxin (Protein)


Gene name mazF Uniprot ID A0A0E0TBF0
Locus tag GT50_RS16200 Protein ID WP_013523721.1
Coordinates 3303731..3304060 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G8N5V3
Locus tag GT50_RS16195 Protein ID WP_011231142.1
Coordinates 3303462..3303731 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GT50_RS16160 3299162..3300142 + 981 WP_053414779.1 ABC transporter permease -
GT50_RS16165 3300211..3300678 - 468 WP_021321939.1 hypothetical protein -
GT50_RS16170 3300692..3301456 - 765 WP_011231140.1 hypothetical protein -
GT50_RS16175 3301583..3302638 + 1056 WP_053414780.1 transposase -
GT50_RS16195 3303462..3303731 + 270 WP_011231142.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GT50_RS16200 3303731..3304060 + 330 WP_013523721.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GT50_RS18755 3304342..3304527 + 186 Protein_3178 pirin family protein -
GT50_RS16205 3304623..3305372 + 750 WP_013523723.1 oxygen-insensitive NADPH nitroreductase -
GT50_RS16210 3305562..3306305 + 744 WP_013523724.1 NAD-dependent protein deacylase -
GT50_RS16215 3306484..3307662 + 1179 WP_013523725.1 acyl-CoA dehydrogenase family protein -
GT50_RS16220 3307699..3308463 + 765 WP_013523726.1 beta-ketoacyl-ACP reductase -
GT50_RS16225 3308482..3308931 + 450 WP_013523727.1 MaoC family dehydratase N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-107)

Antitoxin

Antitoxin PemI-like

(12-87)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12041.83 Da        Isoelectric Point: 7.1331

>T47950 WP_013523721.1 NZ_CP008934:3303731-3304060 [Geobacillus stearothermophilus 10]
MQAPDRGDLVYVNFNPQAGHEQAGKRPGIVLSPKRFNQLTGFAVLCPITRQQKGYPFEVELPSGLAVEGVILTDQVKSLD
WRARQLQIVGRAPNEVVSDCLDLIHAFLS

Download         Length: 330 bp

>T47950 NZ_CP008934:3303731-3304060 [Geobacillus stearothermophilus 10]
ATGCAAGCGCCGGATCGTGGGGATCTTGTTTACGTCAATTTCAATCCACAAGCAGGGCATGAGCAGGCAGGAAAAAGGCC
CGGCATCGTTCTATCACCAAAGCGGTTCAATCAATTGACAGGGTTTGCGGTGCTTTGCCCGATCACCCGGCAACAAAAAG
GATATCCATTTGAAGTGGAATTGCCATCAGGCTTGGCCGTTGAAGGCGTGATTTTAACCGATCAGGTCAAAAGTTTAGAT
TGGCGCGCCCGACAGCTTCAAATAGTAGGACGGGCGCCCAATGAAGTTGTTTCGGATTGCTTGGATCTGATTCATGCTTT
TCTTTCGTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9992.67 Da        Isoelectric Point: 7.2050

>AT47950 WP_011231142.1 NZ_CP008934:3303462-3303731 [Geobacillus stearothermophilus 10]
MELNKQKGVSIMTITVQKWGNSLAVRIPSVIAERLALHQGSEVEMIVENQAIKLIPKKKKPTLEELLAKITPENRHAEID
FGTEGNELF

Download         Length: 270 bp

>AT47950 NZ_CP008934:3303462-3303731 [Geobacillus stearothermophilus 10]
ATGGAATTAAACAAACAGAAAGGAGTGTCAATCATGACAATAACAGTTCAAAAATGGGGAAACAGCCTTGCGGTTCGTAT
CCCAAGCGTGATTGCTGAACGTTTAGCGCTTCATCAAGGATCAGAAGTGGAGATGATCGTTGAGAACCAAGCGATCAAGT
TGATCCCGAAAAAGAAAAAGCCGACATTGGAGGAACTTTTGGCTAAAATCACGCCGGAAAATCGCCATGCTGAAATTGAT
TTTGGAACAGAAGGGAATGAATTGTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0TBF0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9HWH2

References