Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 1998087..1998723 Replicon chromosome
Accession NZ_CP008934
Organism Geobacillus stearothermophilus 10

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag GT50_RS09910 Protein ID WP_003253417.1
Coordinates 1998373..1998723 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0D8C733
Locus tag GT50_RS09905 Protein ID WP_013144044.1
Coordinates 1998087..1998368 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GT50_RS09885 1994338..1994961 - 624 WP_053414273.1 rhomboid family intramembrane serine protease -
GT50_RS09890 1995075..1995464 + 390 WP_011229738.1 holo-ACP synthase -
GT50_RS09895 1995544..1996557 + 1014 WP_013144042.1 outer membrane lipoprotein carrier protein LolA -
GT50_RS09900 1996806..1997960 + 1155 WP_013144043.1 alanine racemase -
GT50_RS09905 1998087..1998368 + 282 WP_013144044.1 hypothetical protein Antitoxin
GT50_RS09910 1998373..1998723 + 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GT50_RS09915 1998989..2001154 + 2166 WP_053414274.1 RNA-binding transcriptional accessory protein -
GT50_RS19035 2001173..2001286 - 114 WP_013144046.1 cortex morphogenetic protein CmpA -
GT50_RS09920 2001376..2001825 + 450 WP_013144047.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T47949 WP_003253417.1 NZ_CP008934:1998373-1998723 [Geobacillus stearothermophilus 10]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T47949 NZ_CP008934:1998373-1998723 [Geobacillus stearothermophilus 10]
TTGATTGTCAAACGTGGCGACGTGTATTTTGCGGACCTTTCCCCGGTTGTTGGCTCGGAGCAGGGCGGCGTGCGCCCCGT
GTTGGTGATCCAAAACGATATCGGCAATCGTTTTAGCCCGACGGTGATTGTCGCGGCGATTACGGCGCAAATCCAAAAAG
CGAAGCTGCCGACGCATGTCGAGATTGACGCGAAACGCTACGGGTTTGAACGCGATTCGGTCATTTTGCTTGAGCAAATT
CGCACGATCGACAAGCAACGGCTGACCGATAAAATCACTCATTTGGACGATGAAATGATGGATAAAGTCGATGAGGCGCT
GCAAATTAGCTTAGGACTGATCGACTTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10669.17 Da        Isoelectric Point: 7.2027

>AT47949 WP_013144044.1 NZ_CP008934:1998087-1998368 [Geobacillus stearothermophilus 10]
VSESGATTEIVVRLPQSLLTELDVLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT47949 NZ_CP008934:1998087-1998368 [Geobacillus stearothermophilus 10]
GTGTCGGAATCTGGCGCAACAACGGAAATCGTCGTTCGTTTGCCGCAGTCGCTGCTGACAGAACTGGACGTGCTCGTAAA
GCAGGAAAACGGCAACCGCAATGAACTCATTTATCAAGCGACGAAAATGTACATTCGCGAGCGGAAGAAACGGCAAATTC
GCGAGGCGATGAGACGAGGCTACATGGAAATGGCGAAAATCAATTTATCTATCGCTTCTGAAGCGTTTCATGCTGAATAC
GAGGCCGACCACACCGTTGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A0D8C733

References