47948

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 224977..225728 Replicon plasmid pPMK1-NDM
Accession NZ_CP008933
Organism Klebsiella pneumoniae strain PMK1

Toxin (Protein)


Gene name ataT Uniprot ID H6U1U8
Locus tag PMK1_RS29045 Protein ID WP_014386536.1
Coordinates 225246..225728 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A071LPN3
Locus tag PMK1_RS29040 Protein ID WP_004902250.1
Coordinates 224977..225255 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PMK1_RS32415 220605..221030 - 426 WP_000522996.1 organomercurial transporter MerC -
PMK1_RS32420 221058..221333 - 276 WP_000732275.1 mercury resistance system periplasmic binding protein MerP -
PMK1_RS31780 221349..221729 - 381 WP_001294654.1 mercuric ion transporter MerT -
PMK1_RS29015 221801..222256 + 456 WP_001166628.1 Hg(II)-responsive transcriptional regulator -
PMK1_RS29020 222909..223367 + 459 WP_014386535.1 hypothetical protein -
PMK1_RS29030 224197..224538 + 342 WP_004902257.1 hypothetical protein -
PMK1_RS29035 224646..224858 + 213 WP_004902255.1 hypothetical protein -
PMK1_RS29040 224977..225255 + 279 WP_004902250.1 DUF1778 domain-containing protein Antitoxin
PMK1_RS29045 225246..225728 + 483 WP_014386536.1 GNAT family N-acetyltransferase Toxin
PMK1_RS29055 226763..227302 + 540 WP_004902239.1 hypothetical protein -
PMK1_RS29060 227407..227799 + 393 WP_032442757.1 hypothetical protein -
PMK1_RS29065 227900..228655 + 756 WP_004902235.1 DUF2971 domain-containing protein -
PMK1_RS29070 228682..229329 + 648 WP_014386537.1 EcsC family protein -
PMK1_RS31790 229461..229730 - 270 Protein_239 kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / blaNDM-1 / qnrB1 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / catA1 htpB / htpB / htpB 1..304526 304526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17662.45 Da        Isoelectric Point: 8.9130

>T47948 WP_014386536.1 NZ_CP008933:225246-225728 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Download         Length: 483 bp

>T47948 NZ_CP008933:225246-225728 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10704.23 Da        Isoelectric Point: 4.5271

>AT47948 WP_004902250.1 NZ_CP008933:224977-225255 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT47948 NZ_CP008933:224977-225255 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBI1

Antitoxin

Source ID Structure
AlphaFold DB A0A071LPN3

References