47947

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 161495..162017 Replicon plasmid pPMK1-NDM
Accession NZ_CP008933
Organism Klebsiella pneumoniae strain PMK1

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag PMK1_RS28700 Protein ID WP_038991638.1
Coordinates 161495..161779 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag PMK1_RS28705 Protein ID WP_004181777.1
Coordinates 161769..162017 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PMK1_RS31350 156695..157790 + 1096 Protein_159 conjugal transfer protein TraG N-terminal domain-containing protein -
PMK1_RS28685 157889..159076 - 1188 WP_040120325.1 transposase -
PMK1_RS31355 159112..159540 + 429 WP_077256229.1 IS200/IS605 family transposase -
PMK1_RS31360 159619..159843 + 225 Protein_162 transposase -
PMK1_RS28695 160115..161341 - 1227 WP_040120327.1 transposase -
PMK1_RS32390 161377..161478 + 102 Protein_164 IS200/IS605 family transposase -
PMK1_RS28700 161495..161779 - 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PMK1_RS28705 161769..162017 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
PMK1_RS28710 162308..164107 + 1800 WP_038991636.1 ATP-dependent helicase -
PMK1_RS28715 164441..165427 + 987 WP_040120328.1 hypothetical protein -
PMK1_RS28720 165572..165925 + 354 WP_004181774.1 hypothetical protein -
PMK1_RS28725 165987..166808 + 822 WP_004181772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / blaNDM-1 / qnrB1 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / catA1 htpB / htpB / htpB 1..304526 304526


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T47947 WP_038991638.1 NZ_CP008933:c161779-161495 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T47947 NZ_CP008933:c161779-161495 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT47947 WP_004181777.1 NZ_CP008933:c162017-161769 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT47947 NZ_CP008933:c162017-161769 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References