Detailed information of TA system TA047946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 5655..6565
• Replicon: plasmid pPMK1-NDM
• Accession: NZ_CP008933
• Organism: Klebsiella pneumoniae strain PMK1

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: PMK1_RS27955
• Protein ID: WP_004026354.1
• Coordinates: 5655..6125 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: PMK1_RS27960
• Protein ID: WP_004181895.1
• Coordinates: 6122..6565 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PMK1_RS27930	1855..2013	-	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
PMK1_RS27935	2085..3197	+	1113	WP_014386573.1	IS481-like element ISKpn28 family transposase	-
PMK1_RS27950	5285..5545	+	261	WP_004026352.1	hypothetical protein	-
PMK1_RS27955	5655..6125	-	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
PMK1_RS27960	6122..6565	-	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
PMK1_RS27965	6666..7937	-	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
PMK1_RS27970	7937..8359	-	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
PMK1_RS27975	8445..9932	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / blaNDM-1 / qnrB1 / dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / catA1	htpB / htpB / htpB	1..304526	304526

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T47946 WP_004026354.1 NZ_CP008933:c6125-5655 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T47946 NZ_CP008933:c6125-5655 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACCCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATACAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAATTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT47946 WP_004181895.1 NZ_CP008933:c6565-6122 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT47946 NZ_CP008933:c6565-6122 [Klebsiella pneumoniae]
ATGAAAACTTTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
TGTAGCGCTCCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGTGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCAACCACATTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTTATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAGGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAAACGGGAGCCTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A2J4RLY9