Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4150..4675 Replicon plasmid pPMK1-C
Accession NZ_CP008932
Organism Klebsiella pneumoniae strain PMK1

Toxin (Protein)


Gene name ccdB Uniprot ID W8UQV6
Locus tag PMK1_RS27570 Protein ID WP_001568026.1
Coordinates 4370..4675 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag PMK1_RS27565 Protein ID WP_001568025.1
Coordinates 4150..4368 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PMK1_RS27535 406..900 - 495 WP_020805591.1 hypothetical protein -
PMK1_RS27540 931..1503 - 573 WP_020805590.1 hypothetical protein -
PMK1_RS27545 1500..1748 - 249 WP_000272716.1 hypothetical protein -
PMK1_RS27550 2185..2874 + 690 WP_020315560.1 RES family NAD+ phosphorylase -
PMK1_RS27555 2906..3595 - 690 WP_004193995.1 hypothetical protein -
PMK1_RS33630 3735..3980 + 246 Protein_6 hypothetical protein -
PMK1_RS27565 4150..4368 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
PMK1_RS27570 4370..4675 + 306 WP_001568026.1 type II toxin-antitoxin system toxin CcdB Toxin
PMK1_RS27575 4844..5239 + 396 WP_072158608.1 hypothetical protein -
PMK1_RS27580 5266..5589 + 324 WP_022644730.1 hypothetical protein -
PMK1_RS27585 5586..6602 + 1017 WP_020315256.1 hypothetical protein -
PMK1_RS27590 6937..8205 - 1269 WP_019705993.1 ISL3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib mrkA / mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..69947 69947
- flank IS/Tn - - 6937..8205 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11645.32 Da        Isoelectric Point: 5.6919

>T47945 WP_001568026.1 NZ_CP008932:4370-4675 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T47945 NZ_CP008932:4370-4675 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT47945 WP_001568025.1 NZ_CP008932:4150-4368 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT47945 NZ_CP008932:4150-4368 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EZN1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References