Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | ccdAB/CcdA(antitoxin) |
Location | 4150..4675 | Replicon | plasmid pPMK1-C |
Accession | NZ_CP008932 | ||
Organism | Klebsiella pneumoniae strain PMK1 |
Toxin (Protein)
Gene name | ccdB | Uniprot ID | W8UQV6 |
Locus tag | PMK1_RS27570 | Protein ID | WP_001568026.1 |
Coordinates | 4370..4675 (+) | Length | 102 a.a. |
Antitoxin (Protein)
Gene name | ccdA | Uniprot ID | W8V2V6 |
Locus tag | PMK1_RS27565 | Protein ID | WP_001568025.1 |
Coordinates | 4150..4368 (+) | Length | 73 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
PMK1_RS27535 | 406..900 | - | 495 | WP_020805591.1 | hypothetical protein | - |
PMK1_RS27540 | 931..1503 | - | 573 | WP_020805590.1 | hypothetical protein | - |
PMK1_RS27545 | 1500..1748 | - | 249 | WP_000272716.1 | hypothetical protein | - |
PMK1_RS27550 | 2185..2874 | + | 690 | WP_020315560.1 | RES family NAD+ phosphorylase | - |
PMK1_RS27555 | 2906..3595 | - | 690 | WP_004193995.1 | hypothetical protein | - |
PMK1_RS33630 | 3735..3980 | + | 246 | Protein_6 | hypothetical protein | - |
PMK1_RS27565 | 4150..4368 | + | 219 | WP_001568025.1 | type II toxin-antitoxin system antitoxin CcdA | Antitoxin |
PMK1_RS27570 | 4370..4675 | + | 306 | WP_001568026.1 | type II toxin-antitoxin system toxin CcdB | Toxin |
PMK1_RS27575 | 4844..5239 | + | 396 | WP_072158608.1 | hypothetical protein | - |
PMK1_RS27580 | 5266..5589 | + | 324 | WP_022644730.1 | hypothetical protein | - |
PMK1_RS27585 | 5586..6602 | + | 1017 | WP_020315256.1 | hypothetical protein | - |
PMK1_RS27590 | 6937..8205 | - | 1269 | WP_019705993.1 | ISL3 family transposase | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | aph(6)-Id / aph(3'')-Ib | mrkA / mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ | 1..69947 | 69947 | |
- | flank | IS/Tn | - | - | 6937..8205 | 1268 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11645.32 Da Isoelectric Point: 5.6919
>T47945 WP_001568026.1 NZ_CP008932:4370-4675 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T47945 NZ_CP008932:4370-4675 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8392.34 Da Isoelectric Point: 4.7405
>AT47945 WP_001568025.1 NZ_CP008932:4150-4368 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT47945 NZ_CP008932:4150-4368 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A514EZN1 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A2J4ZZP8 |