Detailed information of TA system TA047944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 81879..82615
• Replicon: plasmid pPMK1-A
• Accession: NZ_CP008930
• Organism: Klebsiella pneumoniae strain PMK1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: PMK1_RS26335
• Protein ID: WP_003026803.1
• Coordinates: 81879..82361 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: PMK1_RS26340
• Protein ID: WP_003026799.1
• Coordinates: 82349..82615 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PMK1_RS26315	77516..78478	+	963	WP_004152113.1	M48 family metalloprotease	-
PMK1_RS32295	78566..79820	+	1255	Protein_73	IS3 family transposase	-
PMK1_RS26330	80321..81667	-	1347	WP_077256225.1	ISNCY family transposase	-
PMK1_RS26335	81879..82361	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
PMK1_RS26340	82349..82615	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
PMK1_RS26345	82791..83045	-	255	WP_004152108.1	hypothetical protein	-
PMK1_RS26350	83121..83378	-	258	WP_004152107.1	hypothetical protein	-
PMK1_RS26355	83427..83630	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
PMK1_RS26360	83664..84032	-	369	WP_004152105.1	hypothetical protein	-
PMK1_RS26365	84076..84570	-	495	WP_004152104.1	DNA-binding protein	-
PMK1_RS26370	84601..85176	-	576	WP_004152103.1	hypothetical protein	-
PMK1_RS26375	85164..85433	-	270	WP_004152102.1	hypothetical protein	-
PMK1_RS26380	85791..86141	+	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
PMK1_RS26385	86191..86553	+	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(D)	-	1..187571	187571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T47944 WP_003026803.1 NZ_CP008930:c82361-81879 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T47944 NZ_CP008930:c82361-81879 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT47944 WP_003026799.1 NZ_CP008930:c82615-82349 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT47944 NZ_CP008930:c82615-82349 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66