Detailed information of TA system TA047904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 450993..451530
• Replicon: chromosome
• Accession: NZ_CP008909
• Organism: Burkholderia pseudomallei MSHR5848

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: DP65_RS01965
• Protein ID: WP_004549731.1
• Coordinates: 451261..451530 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: DP65_RS01960
• Protein ID: WP_004525420.1
• Coordinates: 450993..451277 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DP65_RS39275	446280..446465	-	186	WP_024429283.1	hypothetical protein	-
DP65_RS01935	446462..448177	-	1716	WP_024429282.1	terminase large subunit	-
DP65_RS01940	448181..448657	-	477	WP_004549538.1	phage terminase small subunit P27 family	-
DP65_RS32105	448795..449151	-	357	WP_024429281.1	HNH endonuclease	-
DP65_RS01950	449240..449818	-	579	WP_038738294.1	hypothetical protein	-
DP65_RS01955	449820..450731	-	912	WP_143281104.1	hypothetical protein	-
DP65_RS01960	450993..451277	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
DP65_RS01965	451261..451530	-	270	WP_004549731.1	BrnT family toxin	Toxin
DP65_RS01970	451579..452022	-	444	WP_004548453.1	hypothetical protein	-
DP65_RS01975	452019..452279	-	261	WP_024429361.1	hypothetical protein	-
DP65_RS32110	452255..452587	-	333	WP_038725178.1	hypothetical protein	-
DP65_RS01980	452617..453015	-	399	WP_038738296.1	DUF1364 family protein	-
DP65_RS01985	453030..453377	-	348	WP_043289604.1	DUF2591 family protein	-
DP65_RS01990	453374..453718	-	345	WP_143297881.1	winged helix-turn-helix domain-containing protein	-
DP65_RS01995	453728..454264	-	537	WP_024429363.1	DUF1064 domain-containing protein	-
DP65_RS02000	454261..455253	-	993	WP_038738301.1	hypothetical protein	-
DP65_RS02005	455407..455667	-	261	WP_004526650.1	helix-turn-helix domain-containing protein	-
DP65_RS02010	455664..456485	-	822	WP_015967391.1	chromosome partitioning protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	406112..518868	112756

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T47904 WP_004549731.1 NZ_CP008909:c451530-451261 [Burkholderia pseudomallei MSHR5848]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T47904 NZ_CP008909:c451530-451261 [Burkholderia pseudomallei MSHR5848]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT47904 WP_004525420.1 NZ_CP008909:c451277-450993 [Burkholderia pseudomallei MSHR5848]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT47904 NZ_CP008909:c451277-450993 [Burkholderia pseudomallei MSHR5848]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: A0A0F6L9S8