Detailed information of TA system TA047903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 67043..67680
• Replicon: plasmid pENT-4bd
• Accession: NZ_CP008907
• Organism: Enterobacter hormaechei subsp. hoffmannii ECR091

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A9E6WXZ4
• Locus tag: ECR091_RS23090
• Protein ID: WP_032662169.1
• Coordinates: 67043..67453 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A9E6WTF5
• Locus tag: ECR091_RS23095
• Protein ID: WP_032662167.1
• Coordinates: 67450..67680 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECR091_RS23070	62408..62758	+	351	WP_032662182.1	DUF305 domain-containing protein	-
ECR091_RS23075	62944..63852	-	909	WP_032662179.1	HNH endonuclease	-
ECR091_RS23080	64400..65422	-	1023	WP_040119796.1	helicase UvrD	-
ECR091_RS23085	65407..66969	-	1563	WP_040119797.1	AAA family ATPase	-
ECR091_RS23090	67043..67453	-	411	WP_032662169.1	PIN domain-containing protein	Toxin
ECR091_RS23095	67450..67680	-	231	WP_032662167.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ECR091_RS25325	68026..71151	+	3126	WP_040119768.1	hypothetical protein	-
ECR091_RS23105	71438..71728	+	291	WP_040119769.1	hypothetical protein	-
ECR091_RS23110	71718..72617	+	900	WP_040119770.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..111227	111227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-127)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14729.03 Da
• Isoelectric Point: 5.7351

>T47903 WP_032662169.1 NZ_CP008907:c67453-67043 [Enterobacter hormaechei subsp. hoffmannii ECR091]
VNKMYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITWAEVSLASQEAGPAAQKLADAFAARLDAILPWDRTAV
DATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVR

Nucleotide

• Length: 411 bp

>T47903 NZ_CP008907:c67453-67043 [Enterobacter hormaechei subsp. hoffmannii ECR091]
GTGAACAAAATGTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTGCGGGGTCACCGCATTGTGGTTTCGGCCATCACCTGGGCCGAAGTGTCACTGGCATCACAGGAAG
CAGGACCAGCCGCACAGAAGCTGGCTGACGCCTTCGCTGCCCGCCTTGATGCCATCCTGCCCTGGGACCGTACCGCGGTG
GACGCGACCACGGAAATTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGGCCGAACGACACGGCGATCGCCGGGCA
CGCCATCGCCGCCGGTGCCGTGCTGGTGACCAATAATGTGCGGGAATTTGAGCGGGTGCCGGGTCTTGTGCTGGAAGACT
GGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8645.77 Da
• Isoelectric Point: 4.4692

>AT47903 WP_032662167.1 NZ_CP008907:c67680-67450 [Enterobacter hormaechei subsp. hoffmannii ECR091]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAERGDVVSDEGRVNL

Nucleotide

• Length: 231 bp

>AT47903 NZ_CP008907:c67680-67450 [Enterobacter hormaechei subsp. hoffmannii ECR091]
ATGAGAACCGTATCCATTTTCAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCCTGGAGCTCG
AAAAAGCTGACCCGGACTTTATGGCTGAGCGCGGGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA