Detailed information of TA system TA047902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 33993..34732
• Replicon: plasmid pENT-4bd
• Accession: NZ_CP008907
• Organism: Enterobacter hormaechei subsp. hoffmannii ECR091

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A9E6WPH7
• Locus tag: ECR091_RS22920
• Protein ID: WP_013087269.1
• Coordinates: 33993..34478 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A9E6WPN8
• Locus tag: ECR091_RS22925
• Protein ID: WP_007869691.1
• Coordinates: 34466..34732 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECR091_RS22900	29145..30221	+	1077	WP_000227970.1	IS110 family transposase	-
ECR091_RS25320	30238..30546	+	309	Protein_34	DDE-type integrase/transposase/recombinase	-
ECR091_RS25010	30590..31928	+	1339	Protein_35	IS3 family transposase	-
ECR091_RS22915	32441..33786	-	1346	Protein_36	ISNCY family transposase	-
ECR091_RS22920	33993..34478	-	486	WP_013087269.1	GNAT family N-acetyltransferase	Toxin
ECR091_RS22925	34466..34732	-	267	WP_007869691.1	DUF1778 domain-containing protein	Antitoxin
ECR091_RS22935	35385..36398	+	1014	WP_040119789.1	replication initiation protein	-
ECR091_RS22940	37288..38091	+	804	WP_032664829.1	DUF4225 domain-containing protein	-
ECR091_RS22945	38069..38392	-	324	WP_040119790.1	hypothetical protein	-
ECR091_RS22950	38407..39177	-	771	WP_032664827.1	conjugal transfer protein TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..111227	111227

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-146)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17736.45 Da
• Isoelectric Point: 9.5181

>T47902 WP_013087269.1 NZ_CP008907:c34478-33993 [Enterobacter hormaechei subsp. hoffmannii ECR091]
VGRVTAPEPLSSSHQLAEFVSGEAVLDDWLKQRGLKNQALGAARTFVVCKRDTKQVVGFYSLATGSVNHVEAMGSLRRNM
PDPIPVIILARLAVDVSYRGQGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYLHHGFKASQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T47902 NZ_CP008907:c34478-33993 [Enterobacter hormaechei subsp. hoffmannii ECR091]
GTGGGACGTGTAACAGCACCAGAACCATTATCCAGCTCCCATCAACTAGCAGAGTTCGTCAGTGGTGAGGCAGTACTTGA
TGACTGGCTTAAACAGAGGGGATTAAAAAACCAAGCTCTTGGTGCAGCACGAACGTTCGTCGTTTGTAAAAGAGACACTA
AACAGGTTGTCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACGTGGAAGCAATGGGAAGCCTCAGGCGCAATATG
CCAGATCCAATTCCCGTAATCATACTTGCTCGCCTGGCAGTTGATGTTTCATACCGTGGCCAAGGGCTCGGGGCCGATTT
ACTGCATGATGCAGTTCTGCGCTGTTACCGTGTAGCTGAGAATATTGGAGTCCGTGCGATTATGGTTCATGCACTGACTG
AAGAAGCCAAAAACTTTTATCTTCACCATGGCTTTAAGGCATCACAAACTCAGGAACGGACATTGTTTTTAAAGTTACCA
CAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10080.46 Da
• Isoelectric Point: 4.3837

>AT47902 WP_007869691.1 NZ_CP008907:c34732-34466 [Enterobacter hormaechei subsp. hoffmannii ECR091]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVSDEAAIDKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT47902 NZ_CP008907:c34732-34466 [Enterobacter hormaechei subsp. hoffmannii ECR091]
ATGAAATCAGATGTTCAGCTCAACCTTAGAGCTAAAGAATCTCAAAGAGCACTTATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACGGACTTCATCCTGGAAATGGCATGTCAGGCTGCGGAGAATGTGATCCTCGATCGTCGCGTATTTAATT
TTAACGATGAGCAGTATGCAGAGTTTATCGATATGCTCGATGCACCGGTTTCGGATGAGGCGGCTATTGATAAACTTCTG
GCAAGGAAACCTCAGTGGGACGTGTAA