Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2820917..2821472 Replicon chromosome
Accession NZ_CP008905
Organism Enterobacter hormaechei subsp. hoffmannii ECR091

Toxin (Protein)


Gene name shpA Uniprot ID A0A839BSP9
Locus tag ECR091_RS24145 Protein ID WP_071881499.1
Coordinates 2821206..2821472 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A839BUZ1
Locus tag ECR091_RS13305 Protein ID WP_038416295.1
Coordinates 2820917..2821219 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECR091_RS13285 2816130..2817206 + 1077 WP_000227970.1 IS110 family transposase -
ECR091_RS24860 2817223..2817510 + 288 Protein_2604 DDE-type integrase/transposase/recombinase -
ECR091_RS13290 2817542..2818246 + 705 Protein_2605 IS5-like element IS5 family transposase -
ECR091_RS13295 2818545..2819456 + 912 WP_038416293.1 acetamidase/formamidase family protein -
ECR091_RS13300 2819453..2820796 + 1344 WP_038416294.1 APC family permease -
ECR091_RS13305 2820917..2821219 - 303 WP_038416295.1 BrnA antitoxin family protein Antitoxin
ECR091_RS24145 2821206..2821472 - 267 WP_071881499.1 BrnT family toxin Toxin
ECR091_RS13315 2821481..2821828 + 348 Protein_2610 transposase -
ECR091_RS25185 2821801..2821875 - 75 Protein_2611 hypothetical protein -
ECR091_RS13320 2821962..2822717 - 756 WP_001290572.1 M48 family metallopeptidase -
ECR091_RS13325 2822714..2825998 - 3285 WP_038416297.1 type I restriction endonuclease subunit R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 2813766..2821915 8149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(9-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10474.91 Da        Isoelectric Point: 10.0832

>T47897 WP_071881499.1 NZ_CP008905:c2821472-2821206 [Enterobacter hormaechei subsp. hoffmannii ECR091]
IRDLFAPSLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLSLVHRIIVIMVAHSVRFESGTEVIRIISARKADSK
ERNRYEHG

Download         Length: 267 bp

>T47897 NZ_CP008905:c2821472-2821206 [Enterobacter hormaechei subsp. hoffmannii ECR091]
ATCAGGGACTTGTTCGCACCTTCCCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGTGCTCGTCTTTGACGATCCCCG
GCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCAGCCTCGTTCATCGGATCATTGTTA
TCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGCGCCCGGAAGGCGGACAGCAAA
GAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11637.21 Da        Isoelectric Point: 9.4429

>AT47897 WP_038416295.1 NZ_CP008905:c2821219-2820917 [Enterobacter hormaechei subsp. hoffmannii ECR091]
MNMVKHKRDNPDTLTTKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT47897 NZ_CP008905:c2821219-2820917 [Enterobacter hormaechei subsp. hoffmannii ECR091]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGACTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BSP9

Antitoxin

Source ID Structure
AlphaFold DB A0A839BUZ1

References