Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 97092..97618 Replicon plasmid pENT-8a4
Accession NZ_CP008899
Organism Enterobacter hormaechei subsp. hoffmannii ECNIH3

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag ECNIH3_RS22745 Protein ID WP_000323025.1
Coordinates 97331..97618 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag ECNIH3_RS22740 Protein ID WP_000534858.1
Coordinates 97092..97331 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH3_RS22720 93420..94658 + 1239 WP_000219087.1 IS110 family transposase -
ECNIH3_RS22730 95134..95706 + 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
ECNIH3_RS22735 95906..96829 + 924 WP_000167917.1 cation diffusion facilitator family transporter -
ECNIH3_RS22740 97092..97331 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
ECNIH3_RS22745 97331..97618 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
ECNIH3_RS25285 97690..97848 + 159 WP_001447866.1 type I toxin-antitoxin system Hok family toxin -
ECNIH3_RS22750 98457..98777 - 321 WP_000332796.1 hypothetical protein -
ECNIH3_RS22755 99059..99262 - 204 WP_001015183.1 hypothetical protein -
ECNIH3_RS22760 99309..99659 - 351 WP_000743059.1 hypothetical protein -
ECNIH3_RS22765 99719..100321 - 603 WP_012695474.1 hypothetical protein -
ECNIH3_RS22770 100417..101361 - 945 WP_000778029.1 DUF5417 domain-containing protein -
ECNIH3_RS26305 101872..102048 + 177 WP_001371930.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mcr-9 / sul2 / blaSHV-12 / catA2 - 1..255013 255013


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T47885 WP_000323025.1 NZ_CP008899:97331-97618 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T47885 NZ_CP008899:97331-97618 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT47885 WP_000534858.1 NZ_CP008899:97092-97331 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT47885 NZ_CP008899:97092-97331 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References