Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 2831567..2832122 Replicon chromosome
Accession NZ_CP008897
Organism Enterobacter hormaechei subsp. hoffmannii ECNIH3

Toxin (Protein)


Gene name shpA Uniprot ID A0A839BSP9
Locus tag ECNIH3_RS24845 Protein ID WP_071881499.1
Coordinates 2831856..2832122 (-) Length 89 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A839BUZ1
Locus tag ECNIH3_RS13350 Protein ID WP_038416295.1
Coordinates 2831567..2831869 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECNIH3_RS13330 2826780..2827856 + 1077 WP_000227970.1 IS110 family transposase -
ECNIH3_RS25675 2827873..2828160 + 288 Protein_2618 DDE-type integrase/transposase/recombinase -
ECNIH3_RS13335 2828192..2828896 + 705 Protein_2619 IS5-like element IS5 family transposase -
ECNIH3_RS13340 2829195..2830106 + 912 WP_038416293.1 acetamidase/formamidase family protein -
ECNIH3_RS13345 2830103..2831446 + 1344 WP_038416294.1 APC family permease -
ECNIH3_RS13350 2831567..2831869 - 303 WP_038416295.1 BrnA antitoxin family protein Antitoxin
ECNIH3_RS24845 2831856..2832122 - 267 WP_071881499.1 BrnT family toxin Toxin
ECNIH3_RS13360 2832131..2832478 + 348 Protein_2624 transposase -
ECNIH3_RS26015 2832451..2832525 - 75 Protein_2625 hypothetical protein -
ECNIH3_RS13365 2832612..2833367 - 756 WP_001290572.1 M48 family metallopeptidase -
ECNIH3_RS13370 2833364..2836648 - 3285 WP_038416297.1 type I restriction endonuclease subunit R -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 2824416..2832565 8149


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(9-81)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 89 a.a.        Molecular weight: 10474.91 Da        Isoelectric Point: 10.0832

>T47881 WP_071881499.1 NZ_CP008897:c2832122-2831856 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
IRDLFAPSLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLSLVHRIIVIMVAHSVRFESGTEVIRIISARKADSK
ERNRYEHG

Download         Length: 267 bp

>T47881 NZ_CP008897:c2832122-2831856 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
ATCAGGGACTTGTTCGCACCTTCCCTAAGAAAACATGGAATCCGGTTTGAAGAAGCGGTGCTCGTCTTTGACGATCCCCG
GCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCAGCCTCGTTCATCGGATCATTGTTA
TCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGCGCCCGGAAGGCGGACAGCAAA
GAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11637.21 Da        Isoelectric Point: 9.4429

>AT47881 WP_038416295.1 NZ_CP008897:c2831869-2831567 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
MNMVKHKRDNPDTLTTKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT47881 NZ_CP008897:c2831869-2831567 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGACTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A839BSP9

Antitoxin

Source ID Structure
AlphaFold DB A0A839BUZ1

References