Detailed information of TA system TA047872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2266386..2267125
• Replicon: chromosome
• Accession: NZ_CP008897
• Organism: Enterobacter hormaechei subsp. hoffmannii ECNIH3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: ECNIH3_RS10680
• Protein ID: WP_038416064.1
• Coordinates: 2266386..2266871 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A837FCR9
• Locus tag: ECNIH3_RS10685
• Protein ID: WP_003857131.1
• Coordinates: 2266859..2267125 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECNIH3_RS24675	2262074..2262418	-	345	WP_080470381.1	MFS transporter	-
ECNIH3_RS10660	2262415..2263383	-	969	WP_013815099.1	IS5-like element IS903B family transposase	-
ECNIH3_RS10665	2263415..2263786	-	372	Protein_2084	IS5/IS1182 family transposase	-
ECNIH3_RS10670	2263861..2264493	+	633	WP_001567369.1	hypothetical protein	-
ECNIH3_RS10675	2264522..2265925	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
ECNIH3_RS10680	2266386..2266871	-	486	WP_038416064.1	GNAT family N-acetyltransferase	Toxin
ECNIH3_RS10685	2266859..2267125	-	267	WP_003857131.1	DUF1778 domain-containing protein	Antitoxin
ECNIH3_RS10690	2267189..2268118	-	930	WP_038416065.1	LysR family transcriptional regulator	-
ECNIH3_RS10695	2268248..2269636	+	1389	WP_038416066.1	MFS transporter	-
ECNIH3_RS10700	2269615..2270172	-	558	WP_023300131.1	OmpH family outer membrane protein	-
ECNIH3_RS10705	2270293..2271429	-	1137	WP_038416067.1	type 1 fimbrial protein	-
ECNIH3_RS10710	2271453..2271977	-	525	WP_087878309.1	fimbrial protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2247462..2267125	19663

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17546.28 Da
• Isoelectric Point: 9.9308

>T47872 WP_038416064.1 NZ_CP008897:c2266871-2266386 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
VGRVTAPEPLSSVHQLAEFVSGEAVLDEWLKQRGLKNQALAAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSIRGKGLGADLLQDAVLRCYRVAENIGVRAIMVHALTEDAKAFYIHHGFKASQTQERTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T47872 NZ_CP008897:c2266871-2266386 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
GTGGGACGTGTAACCGCCCCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGTGGAGAGGCCGTACTTGA
CGAATGGTTAAAACAAAGAGGTCTGAAAAACCAGGCGCTTGCCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACAA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGGAAGCGACAGGTAATTTGCGGCGTAACATG
CCGGATCCCATTCCCGTGATTATTCTTGCCCGTCTGGCTGTCGATGTCTCAATACGCGGAAAAGGGTTGGGAGCCGATTT
ACTCCAGGATGCGGTTTTACGCTGTTATCGGGTAGCAGAGAATATTGGGGTGCGGGCGATCATGGTTCATGCACTCACTG
AGGACGCCAAAGCGTTTTATATCCACCATGGTTTTAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.50 Da
• Isoelectric Point: 4.3837

>AT47872 WP_003857131.1 NZ_CP008897:c2267125-2266859 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIEKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT47872 NZ_CP008897:c2267125-2266859 [Enterobacter hormaechei subsp. hoffmannii ECNIH3]
ATGAAATCAGATGTTCAACTCAATCTTAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGTACTGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAAAATGTGATCCTCGATCGGCGTGTATTCAATT
TTAACGATGAACAGTATGCAGAGTTCATTGATATGCTTGATGCCCCGGTCGCCGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A837FCR9