Detailed information of TA system TA047867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 2939895..2940336
• Replicon: chromosome
• Accession: NZ_CP008893
• Organism: Burkholderia pseudomallei HBPUB10303a

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: DR54_RS30645
• Protein ID: WP_009939402.1
• Coordinates: 2939895..2940068 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: DR54_RS11820
• Protein ID: WP_004525420.1
• Coordinates: 2940052..2940336 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR54_RS40260	2935494..2935922	-	429	Protein_2356	DDE-type integrase/transposase/recombinase	-
DR54_RS40265	2935920..2936693	-	774	Protein_2357	prohibitin family protein	-
DR54_RS11785	2936690..2936839	-	150	WP_004537646.1	bacteriophage protein Gp44	-
DR54_RS11790	2937089..2937679	+	591	WP_009946442.1	hypothetical protein	-
DR54_RS39790	2937854..2938072	-	219	WP_004549687.1	hypothetical protein	-
DR54_RS11800	2938120..2938467	-	348	WP_009946438.1	hypothetical protein	-
DR54_RS40270	2938496..2938672	-	177	WP_004552958.1	hypothetical protein	-
DR54_RS11815	2938952..2939227	-	276	WP_004537565.1	bacteriophage protein Gp49	-
DR54_RS30645	2939895..2940068	+	174	WP_009939402.1	BrnT family toxin	Toxin
DR54_RS11820	2940052..2940336	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
DR54_RS11830	2940983..2941366	+	384	Protein_2366	DDE-type integrase/transposase/recombinase	-
DR54_RS34340	2941329..2941757	+	429	Protein_2367	IS110 family transposase	-
DR54_RS11835	2941984..2942100	-	117	Protein_2368	IS3 family transposase	-
DR54_RS11840	2942366..2943826	+	1461	WP_004547073.1	metallophosphoesterase	-
DR54_RS11845	2943985..2945247	+	1263	WP_004537187.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2933183..2947475	14292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T47867 WP_009939402.1 NZ_CP008893:2939895-2940068 [Burkholderia pseudomallei HBPUB10303a]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T47867 NZ_CP008893:2939895-2940068 [Burkholderia pseudomallei HBPUB10303a]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT47867 WP_004525420.1 NZ_CP008893:2940052-2940336 [Burkholderia pseudomallei HBPUB10303a]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT47867 NZ_CP008893:2940052-2940336 [Burkholderia pseudomallei HBPUB10303a]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8