Detailed information of TA system TA047864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2212377..2214136
• Replicon: chromosome
• Accession: NZ_CP008891
• Organism: Burkholderia pseudomallei MSHR5858

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B1H781
• Locus tag: DR56_RS08780
• Protein ID: WP_004525276.1
• Coordinates: 2212784..2214136 (+)
• Length: 451 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: Q3JKV9
• Locus tag: DR56_RS08775
• Protein ID: WP_004528870.1
• Coordinates: 2212377..2212787 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR56_RS08765	2208914..2211310	-	2397	WP_011205645.1	cellulose biosynthesis cyclic di-GMP-binding regulatory protein BcsB	-
DR56_RS39400	2211776..2212039	+	264	WP_004199789.1	hypothetical protein	-
DR56_RS08775	2212377..2212787	+	411	WP_004528870.1	helix-turn-helix domain-containing protein	Antitoxin
DR56_RS08780	2212784..2214136	+	1353	WP_004525276.1	type II toxin-antitoxin system HipA family toxin	Toxin
DR56_RS08785	2214304..2214756	-	453	WP_024428481.1	hypothetical protein	-
DR56_RS08795	2215160..2216074	-	915	WP_024428483.1	LysR family transcriptional regulator	-
DR56_RS08800	2216241..2217194	+	954	WP_004187785.1	agmatinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (158-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-71)

Sequences

Toxin

Protein

• Length: 451 a.a.
• Molecular weight: 49790.80 Da
• Isoelectric Point: 9.8300

>T47864 WP_004525276.1 NZ_CP008891:2212784-2214136 [Burkholderia pseudomallei MSHR5858]
VSARRARATRLHLWMNGLPVGYWEHARDGERLVYFDEWIGDPQGRPLSLSLPFTPGNQPYRGRLVSDYFDNLLPDSEPIR
RRIAMRYRTGGTSAFALLATLGRDCVGALQMLPPDEAPDDIERIRGHALADADIARLLREVTSAPQAGRHAPLDDLRLSI
AGAQEKTALLRHRGRWLLPEGSTPTTHILKLPLGLVGNRRADMRTSVENEWLCARIVAAYGLPVARCDIAQFDDQKALVV
ERFDRRPSRDARWLLRLPQEDMCQATGTSALDKYQADGGPGIETIMEVLAGSEHARDDRRAFFAAQIVFWLLAATDGHAK
NFSIAHLPGNRYRSTPLYDVLSAHPVIGRGANQLPAQRARLAMGVRGKHIHYPLHQIRRRHWIAQGQRVGFAPADVDALI
DTLTARTAGVVDAVSARLPRDFPRDVADAIFSGMLGLSARLAGDAAARAP

Nucleotide

• Length: 1353 bp

>T47864 NZ_CP008891:2212784-2214136 [Burkholderia pseudomallei MSHR5858]
GTGAGCGCCCGCCGCGCACGCGCGACGCGCCTGCACCTGTGGATGAACGGCCTGCCCGTCGGCTACTGGGAGCACGCGCG
CGACGGCGAGCGCCTTGTCTACTTCGACGAATGGATCGGCGATCCGCAAGGCCGGCCGCTGTCGCTGTCGCTGCCGTTCA
CGCCGGGCAACCAGCCGTATCGCGGTCGGCTCGTCAGCGATTATTTCGACAACCTGCTGCCCGACAGCGAGCCGATCCGC
CGGCGAATCGCGATGCGCTACCGCACGGGCGGCACGTCCGCGTTCGCGCTGCTCGCGACGCTCGGCCGCGATTGCGTCGG
CGCGCTGCAGATGCTGCCGCCCGACGAAGCGCCGGACGACATCGAACGCATCCGCGGCCACGCGCTCGCCGACGCGGACA
TCGCGCGCCTGCTGCGCGAAGTCACGTCCGCGCCGCAGGCCGGCCGGCACGCGCCGCTCGACGATCTGCGCCTGTCGATC
GCCGGCGCGCAGGAGAAGACCGCGCTGCTGCGCCATCGCGGCCGCTGGCTGCTGCCCGAAGGGAGCACGCCGACCACGCA
CATCCTGAAGCTGCCGCTCGGGCTCGTCGGCAACCGGCGCGCCGACATGCGCACGTCGGTCGAGAACGAATGGCTGTGCG
CGCGGATCGTTGCCGCGTACGGGTTGCCCGTCGCGCGCTGCGACATCGCGCAGTTCGACGATCAGAAAGCGCTCGTCGTC
GAGCGCTTCGACCGCCGGCCGTCGCGCGACGCGCGCTGGCTCCTGCGGCTGCCGCAGGAAGACATGTGCCAGGCAACCGG
CACGTCCGCGCTCGACAAATATCAGGCCGACGGCGGCCCCGGCATCGAGACGATCATGGAAGTGCTCGCCGGCTCCGAGC
ACGCGCGGGACGACCGCCGCGCGTTCTTCGCGGCGCAGATCGTGTTCTGGCTGCTCGCCGCGACCGACGGCCACGCGAAG
AACTTCAGCATCGCGCACCTGCCCGGCAACCGCTACCGTTCGACGCCGCTTTACGACGTGCTGTCCGCGCATCCGGTCAT
CGGCCGGGGCGCGAACCAGTTGCCCGCGCAGCGCGCGCGGCTCGCGATGGGCGTGCGCGGCAAGCACATCCACTATCCGC
TGCACCAGATCCGGCGGCGGCACTGGATCGCGCAGGGCCAGCGCGTCGGCTTCGCGCCCGCCGACGTCGACGCGCTGATC
GACACGCTGACCGCGCGCACCGCGGGCGTCGTCGACGCGGTGTCGGCGCGGCTGCCGCGCGATTTTCCGCGCGACGTCGC
CGATGCGATCTTCAGCGGAATGCTCGGCCTGAGCGCAAGGCTCGCCGGCGACGCGGCCGCGCGCGCGCCATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14490.84 Da
• Isoelectric Point: 11.8540

>AT47864 WP_004528870.1 NZ_CP008891:2212377-2212787 [Burkholderia pseudomallei MSHR5858]
MAILIEHEIKTLGQLRPILRGFRKSAGLTQAILASRLGVTQQTYAQFEANPASASVERLFKVLRALDIELTLTLTQVYAA
PAGKDKGEVAKTAAGARARAGARRAVPPASAPAPSAAGRAPRPARKRAAPKKREDW

Nucleotide

• Length: 411 bp

>AT47864 NZ_CP008891:2212377-2212787 [Burkholderia pseudomallei MSHR5858]
ATGGCCATCCTCATCGAGCACGAGATCAAGACACTCGGCCAGCTGCGGCCGATTCTGCGCGGCTTCCGCAAATCGGCCGG
ATTGACGCAGGCGATACTCGCGAGCCGCCTCGGCGTCACGCAGCAGACCTACGCGCAGTTCGAGGCGAACCCGGCGTCGG
CGAGCGTCGAGCGGCTGTTCAAGGTGCTGCGCGCGCTCGACATCGAACTCACGCTCACGCTCACGCAGGTCTACGCCGCG
CCCGCGGGCAAGGACAAGGGCGAGGTTGCGAAGACGGCCGCAGGCGCGCGCGCGCGCGCGGGCGCGCGACGTGCCGTGCC
GCCCGCGTCCGCGCCCGCTCCGAGCGCCGCCGGGCGCGCGCCCCGCCCCGCCCGCAAGCGCGCCGCCCCGAAAAAGCGGG
AGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2K3EM91