Detailed information of TA system TA047862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 630597..631038
• Replicon: chromosome
• Accession: NZ_CP008891
• Organism: Burkholderia pseudomallei MSHR5858

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: DR56_RS30305
• Protein ID: WP_009939402.1
• Coordinates: 630597..630770 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: DR56_RS02560
• Protein ID: WP_004525420.1
• Coordinates: 630754..631038 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR56_RS39875	626196..626624	-	429	Protein_500	DDE-type integrase/transposase/recombinase	-
DR56_RS39880	626622..627395	-	774	Protein_501	prohibitin family protein	-
DR56_RS02520	627392..627541	-	150	WP_004537646.1	bacteriophage protein Gp44	-
DR56_RS02525	627791..628381	+	591	WP_009946442.1	hypothetical protein	-
DR56_RS39225	628556..628774	-	219	WP_004549687.1	hypothetical protein	-
DR56_RS02535	628822..629169	-	348	WP_009946438.1	hypothetical protein	-
DR56_RS39885	629198..629374	-	177	WP_004552958.1	hypothetical protein	-
DR56_RS02550	629654..629929	-	276	WP_025988352.1	hypothetical protein	-
DR56_RS30305	630597..630770	+	174	WP_009939402.1	BrnT family toxin	Toxin
DR56_RS02560	630754..631038	+	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
DR56_RS02570	631685..632068	+	384	Protein_510	DDE-type integrase/transposase/recombinase	-
DR56_RS31165	632031..632459	+	429	Protein_511	IS110 family transposase	-
DR56_RS02575	632686..632802	-	117	Protein_512	IS3 family transposase	-
DR56_RS02580	633068..634528	+	1461	WP_004536920.1	metallophosphoesterase	-
DR56_RS02585	634687..635949	+	1263	WP_004537187.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	623885..638177	14292

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T47862 WP_009939402.1 NZ_CP008891:630597-630770 [Burkholderia pseudomallei MSHR5858]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T47862 NZ_CP008891:630597-630770 [Burkholderia pseudomallei MSHR5858]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT47862 WP_004525420.1 NZ_CP008891:630754-631038 [Burkholderia pseudomallei MSHR5858]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT47862 NZ_CP008891:630754-631038 [Burkholderia pseudomallei MSHR5858]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8