Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5175277..5175824 Replicon chromosome
Accession NZ_CP008886
Organism Raoultella ornithinolytica strain A14

Toxin (Protein)


Gene name ccdB Uniprot ID A0A808XUV0
Locus tag HY59_RS25735 Protein ID WP_048025274.1
Coordinates 5175516..5175824 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A808XYT1
Locus tag HY59_RS23910 Protein ID WP_004869059.1
Coordinates 5175277..5175513 (+) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HY59_RS23895 5171483..5172646 + 1164 WP_044350982.1 multidrug effflux MFS transporter -
HY59_RS23900 5172811..5173713 + 903 WP_032718425.1 N-acetylmuramic acid 6-phosphate etherase -
HY59_RS23905 5173738..5175171 + 1434 WP_004869058.1 PTS N-acetylmuramic acid transporter subunit IIBC -
HY59_RS23910 5175277..5175513 + 237 WP_004869059.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HY59_RS25735 5175516..5175824 + 309 WP_048025274.1 CcdB family protein Toxin
HY59_RS23915 5175940..5176842 + 903 WP_015585496.1 EamA family transporter -
HY59_RS23920 5176951..5177226 + 276 WP_162098187.1 hypothetical protein -
HY59_RS23925 5177321..5178262 - 942 WP_015585487.1 LacI family transcriptional regulator -
HY59_RS23930 5178557..5178871 + 315 WP_004869063.1 PTS sugar transporter subunit IIB -
HY59_RS23935 5178883..5180208 + 1326 WP_004869064.1 PTS cellobiose transporter subunit IIC -
HY59_RS23940 5180343..5180645 + 303 WP_004869065.1 PTS lactose/cellobiose transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11778.62 Da        Isoelectric Point: 6.7156

>T47860 WP_048025274.1 NZ_CP008886:5175516-5175824 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Download         Length: 309 bp

>T47860 NZ_CP008886:5175516-5175824 [Raoultella ornithinolytica]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGGTCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGTCCGCGCGCCGATCGTTTAACACCAG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAACGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTTTGACGGGATTTAA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8898.08 Da        Isoelectric Point: 5.6579

>AT47860 WP_004869059.1 NZ_CP008886:5175277-5175513 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT47860 NZ_CP008886:5175277-5175513 [Raoultella ornithinolytica]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808XUV0

Antitoxin

Source ID Structure
AlphaFold DB A0A808XYT1

References