Detailed information of TA system TA047847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 535718..536297
• Replicon: chromosome
• Accession: NZ_CP008886
• Organism: Raoultella ornithinolytica strain A14

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A808YBH6
• Locus tag: HY59_RS02450
• Protein ID: WP_032716505.1
• Coordinates: 535718..536005 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HY59_RS02455
• Protein ID: WP_004857518.1
• Coordinates: 535992..536297 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HY59_RS02420	531213..531731	-	519	WP_015585229.1	hypothetical protein	-
HY59_RS02425	531728..532540	-	813	WP_004857492.1	winged helix-turn-helix domain-containing protein	-
HY59_RS02430	532684..533262	-	579	WP_004857495.1	TetR/AcrR family transcriptional regulator	-
HY59_RS02435	533410..534192	-	783	WP_048024655.1	NAD(P)H-dependent oxidoreductase	-
HY59_RS02440	534280..535188	+	909	WP_004857513.1	LysR family transcriptional regulator	-
HY59_RS02445	535182..535601	+	420	WP_015585224.1	FosA family fosfomycin resistance glutathione transferase	-
HY59_RS02450	535718..536005	+	288	WP_032716505.1	BrnT family toxin	Toxin
HY59_RS02455	535992..536297	+	306	WP_004857518.1	BrnA antitoxin family protein	Antitoxin
HY59_RS02460	536453..537865	-	1413	WP_015585221.1	PLP-dependent aminotransferase family protein	-
HY59_RS02465	538043..538207	+	165	WP_004857521.1	DUF1127 domain-containing protein	-
HY59_RS02470	538414..538878	+	465	WP_004857523.1	MarR family transcriptional regulator	-
HY59_RS02475	538885..539952	+	1068	WP_004857525.1	HlyD family secretion protein	-
HY59_RS02480	539942..541006	+	1065	WP_004857527.1	DUF2955 domain-containing protein	-
HY59_RS02485	541003..541257	-	255	WP_004857528.1	DUF3343 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (35-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11131.57 Da
• Isoelectric Point: 7.5235

>T47847 WP_032716505.1 NZ_CP008886:535718-536005 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHDRYENGEYRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKKERSRYEHG

Nucleotide

• Length: 288 bp

>T47847 NZ_CP008886:535718-536005 [Raoultella ornithinolytica]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTGACGATCCGCAGCATCTTTCCCGACATGATCGCTATGAAAATGGTGAGTATCGATGGCAAACCATCGGGC
TTGTTCATGGCGTGATTGTTGTTCTTGTGGCGCATTCGATCCGATTTGAAAGTGGAACGGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAAAAAAGAGAGGAGTCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11681.22 Da
• Isoelectric Point: 9.4429

>AT47847 WP_004857518.1 NZ_CP008886:535992-536297 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALAKKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIREQNKKS

Nucleotide

• Length: 306 bp

>AT47847 NZ_CP008886:535992-536297 [Raoultella ornithinolytica]
ATGAGCATGGTTAAACATAAACGCGATAACCCGACAGCGCTTACCGCTCAGCGTGAAGACGAACTCAAAGCGCTGGCGAA
AAAATCAGATGATGACATTGATTACAGCGATATCCCCTCTTCGGACGATGAGCCGTGGTCAGAGGCGGTTCGTGGTAAAT
TTTATCGGCCATTAAAAACGCAGGCCTCTGTGCGCATCGATGCAGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAAACGCGGCTGAATGCGATTTTAAGAGAAGCGATGATTCGCGAGCAGAATAAGAAATCGTAG

Structures

Toxin

• AlphaFold DB: A0A808YBH6