Detailed information of TA system TA047844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/RelB(antitoxin)
• Location: 1040631..1041267
• Replicon: chromosome
• Accession: NZ_CP008885
• Organism: Bifidobacterium longum strain BXY01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D6ZTX4
• Locus tag: GS08_RS04370
• Protein ID: WP_013140632.1
• Coordinates: 1040631..1040987 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GS08_RS04375
• Protein ID: WP_013140633.1
• Coordinates: 1040974..1041267 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS08_RS04340	1035901..1036893	+	993	WP_013140628.1	ABC transporter ATP-binding protein	-
GS08_RS04345	1037092..1037718	+	627	WP_007052130.1	30S ribosomal protein S4	-
GS08_RS04350	1037898..1038680	-	783	WP_013140629.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-
GS08_RS04355	1038818..1039303	-	486	WP_013140630.1	cytidine deaminase	-
GS08_RS04360	1039394..1039747	+	354	Protein_850	SdpI family protein	-
GS08_RS04365	1039852..1040454	+	603	WP_003829233.1	transglutaminase family protein	-
GS08_RS04370	1040631..1040987	-	357	WP_013140632.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GS08_RS04375	1040974..1041267	-	294	WP_013140633.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GS08_RS04380	1041340..1041627	-	288	Protein_854	IS30 family transposase	-
GS08_RS04385	1041859..1042533	+	675	WP_013140635.1	histidine phosphatase family protein	-
GS08_RS04390	1042616..1043014	+	399	WP_007052133.1	DUF948 domain-containing protein	-
GS08_RS11630	1043014..1043250	+	237	WP_007053386.1	hypothetical protein	-
GS08_RS04400	1043379..1046057	+	2679	WP_038425923.1	alanine--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-110)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (11-53)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13263.16 Da
• Isoelectric Point: 7.9649

>T47844 WP_013140632.1 NZ_CP008885:c1040987-1040631 [Bifidobacterium longum]
MMKTDPRQFEIWWVPFAFPDKPSKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGSVAWAINELYPGLLA

Nucleotide

• Length: 357 bp

>T47844 NZ_CP008885:c1040987-1040631 [Bifidobacterium longum]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAAGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCAGCGTCGCCTG
GGCGATCAACGAACTCTACCCGGGCCTACTAGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10865.36 Da
• Isoelectric Point: 4.4919

>AT47844 WP_013140633.1 NZ_CP008885:c1041267-1040974 [Bifidobacterium longum]
MAIKEKATISLDAQTKRDGIAILDTMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFKKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Nucleotide

• Length: 294 bp

>AT47844 NZ_CP008885:c1041267-1040974 [Bifidobacterium longum]
ATGGCTATCAAGGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACACCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCAAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Structures

Toxin

• AlphaFold DB: A0A7U4H5H9