Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemK-mazE/MazF(toxin)
Location 347291..347921 Replicon chromosome
Accession NZ_CP008876
Organism Terribacillus goriensis strain MP602

Toxin (Protein)


Gene name pemK Uniprot ID A0A1H8IGW1
Locus tag GZ22_RS01795 Protein ID WP_038558138.1
Coordinates 347571..347921 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1H8IGZ0
Locus tag GZ22_RS01790 Protein ID WP_038558136.1
Coordinates 347291..347566 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GZ22_RS01770 342872..343237 + 366 WP_038558127.1 holo-ACP synthase -
GZ22_RS01775 343299..344810 + 1512 WP_038558129.1 bifunctional ADP-dependent NAD(P)H-hydrate dehydratase/NAD(P)H-hydrate epimerase -
GZ22_RS01780 344861..345877 + 1017 WP_038558132.1 outer membrane lipoprotein carrier protein LolA -
GZ22_RS01785 345962..347134 + 1173 WP_038558134.1 alanine racemase -
GZ22_RS01790 347291..347566 + 276 WP_038558136.1 hypothetical protein Antitoxin
GZ22_RS01795 347571..347921 + 351 WP_038558138.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GZ22_RS01800 348129..349001 + 873 WP_038558140.1 RsbT co-antagonist protein RsbRA -
GZ22_RS01805 348998..349354 + 357 WP_038558142.1 STAS domain-containing protein -
GZ22_RS01810 349357..349758 + 402 WP_038558144.1 anti-sigma regulatory factor -
GZ22_RS01815 349775..350785 + 1011 WP_038558146.1 PP2C family protein-serine/threonine phosphatase -
GZ22_RS01820 350843..351172 + 330 WP_038558149.1 anti-sigma factor antagonist -
GZ22_RS01825 351172..351651 + 480 WP_170937657.1 anti-sigma B factor RsbW -
GZ22_RS01830 351623..352414 + 792 WP_038558153.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.09 Da        Isoelectric Point: 5.8701

>T47842 WP_038558138.1 NZ_CP008876:347571-347921 [Terribacillus goriensis]
MIVKRGEVYFADLSPVVGSEQGGVRPVLVLQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITQLDPPMMENINRALEISLGLTEF

Download         Length: 351 bp

>T47842 NZ_CP008876:347571-347921 [Terribacillus goriensis]
TTGATCGTAAAAAGAGGCGAAGTCTATTTCGCCGACCTGTCCCCGGTAGTGGGATCAGAACAGGGGGGCGTGCGCCCGGT
ATTGGTCCTGCAGAATGATATCGGAAACCGATTCAGTCCGACCGTCATCATAGCGGCGATAACGGCACAGATCCAAAAAG
CCAAGCTGCCGACCCACGTGGAGATTGATGCCAAGAAGTATGGCTTTGAACGGGATTCCGTCATTCTTCTTGAACAGATC
CGAACAATCGACAAGCAGCGGCTGACAGATAAGATCACACAGCTAGACCCTCCAATGATGGAGAATATCAATCGAGCACT
TGAAATAAGTCTAGGCCTTACAGAGTTCTAG

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10420.89 Da        Isoelectric Point: 4.6512

>AT47842 WP_038558136.1 NZ_CP008876:347291-347566 [Terribacillus goriensis]
MVVSEGLQEIVVKLPKKLLNEVDGLMQNNNNSLDHFFYQAAKSYVQERKNIRETMQKGYMEMARINLNIASEAFQAEEEA
EVTLERLVSGV

Download         Length: 276 bp

>AT47842 NZ_CP008876:347291-347566 [Terribacillus goriensis]
ATGGTTGTGTCCGAAGGCTTACAAGAAATCGTTGTAAAGCTGCCTAAGAAACTCCTGAATGAGGTGGATGGTTTGATGCA
GAACAATAACAATAGTCTCGATCATTTCTTCTATCAAGCGGCTAAGTCATACGTGCAGGAAAGAAAGAATATCCGTGAGA
CCATGCAGAAAGGGTACATGGAAATGGCACGTATCAATCTAAATATCGCTTCAGAAGCTTTCCAAGCTGAAGAGGAGGCA
GAAGTTACGTTAGAGCGCCTTGTAAGCGGGGTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1H8IGW1

Antitoxin

Source ID Structure
AlphaFold DB A0A1H8IGZ0

References