Detailed information of TA system TA047840

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 5515388..5516430
• Replicon: chromosome
• Accession: NZ_CP008873
• Organism: Pseudomonas aeruginosa strain F9670

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HW11_RS25150
• Protein ID: WP_003109777.1
• Coordinates: 5515388..5515963 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: HW11_RS25155
• Protein ID: WP_003050245.1
• Coordinates: 5515960..5516430 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW11_RS25125	5511827..5512552	-	726	WP_003120003.1	CBASS effector endonuclease NucC	-
HW11_RS25130	5512591..5513493	-	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
HW11_RS25135	5513493..5513992	-	500	Protein_5033	HORMA domain containing protein	-
HW11_RS25140	5513989..5514459	-	471	WP_003105626.1	hypothetical protein	-
HW11_RS25145	5514456..5515370	-	915	WP_003120002.1	AAA family ATPase	-
HW11_RS25150	5515388..5515963	-	576	WP_003109777.1	PIN domain-containing protein	Toxin
HW11_RS25155	5515960..5516430	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
HW11_RS25160	5516634..5517017	+	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
HW11_RS25165	5517014..5517247	+	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
HW11_RS25170	5517264..5517623	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
HW11_RS25175	5517635..5518033	+	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
HW11_RS25180	5518030..5518722	+	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
HW11_RS25185	5518719..5519630	+	912	WP_010791730.1	TIGR03749 family integrating conjugative element protein	-
HW11_RS25190	5519620..5521038	+	1419	WP_003119999.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5469280..5587900	118620

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T47840 WP_003109777.1 NZ_CP008873:c5515963-5515388 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T47840 NZ_CP008873:c5515963-5515388 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT47840 WP_003050245.1 NZ_CP008873:c5516430-5515960 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT47840 NZ_CP008873:c5516430-5515960 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68