Detailed information of TA system TA047816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3532020..3533062
• Replicon: chromosome
• Accession: NZ_CP008870
• Organism: Pseudomonas aeruginosa strain W36662

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HV97_RS16585
• Protein ID: WP_003050248.1
• Coordinates: 3532020..3532595 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: HV97_RS16590
• Protein ID: WP_003050245.1
• Coordinates: 3532592..3533062 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV97_RS16560	3528460..3529185	-	726	WP_023087304.1	CBASS effector endonuclease NucC	-
HV97_RS16565	3529222..3530124	-	903	WP_023087303.1	nucleotidyltransferase domain-containing protein	-
HV97_RS16570	3530124..3530624	-	501	WP_003050262.1	hypothetical protein	-
HV97_RS16575	3530621..3531091	-	471	WP_023087302.1	hypothetical protein	-
HV97_RS16580	3531088..3532002	-	915	WP_023087301.1	AAA family ATPase	-
HV97_RS16585	3532020..3532595	-	576	WP_003050248.1	PIN domain-containing protein	Toxin
HV97_RS16590	3532592..3533062	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
HV97_RS16595	3533266..3533649	+	384	WP_023087300.1	RAQPRD family integrative conjugative element protein	-
HV97_RS16600	3533646..3533879	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
HV97_RS16605	3533896..3534255	+	360	WP_021195311.1	TIGR03745 family integrating conjugative element membrane protein	-
HV97_RS16610	3534268..3534666	+	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
HV97_RS16615	3534663..3535355	+	693	WP_023087299.1	TIGR03746 family integrating conjugative element protein	-
HV97_RS16620	3535352..3536263	+	912	WP_023087298.1	TIGR03749 family integrating conjugative element protein	-
HV97_RS16625	3536253..3537671	+	1419	WP_023087297.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3486532..3601748	115216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21630.76 Da
• Isoelectric Point: 5.6130

>T47816 WP_003050248.1 NZ_CP008870:c3532595-3532020 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T47816 NZ_CP008870:c3532595-3532020 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT47816 WP_003050245.1 NZ_CP008870:c3533062-3532592 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT47816 NZ_CP008870:c3533062-3532592 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCGTTGCGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68