Detailed information of TA system TA047810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5469319..5469905
• Replicon: chromosome
• Accession: NZ_CP008869
• Organism: Pseudomonas aeruginosa strain W16407

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HV98_RS26015
• Protein ID: WP_003120987.1
• Coordinates: 5469606..5469905 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HV98_RS26010
• Protein ID: WP_003448662.1
• Coordinates: 5469319..5469609 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV98_RS25995	5464903..5466792	+	1890	WP_023101908.1	hypothetical protein	-
HV98_RS26000	5466789..5468765	+	1977	WP_031691363.1	DEAD/DEAH box helicase	-
HV98_RS26005	5468904..5469248	+	345	WP_023082045.1	hypothetical protein	-
HV98_RS26010	5469319..5469609	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HV98_RS26015	5469606..5469905	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV98_RS26020	5470107..5471231	+	1125	WP_023082046.1	TcpQ domain-containing protein	-
HV98_RS26025	5471231..5472940	+	1710	WP_010792227.1	PilN family type IVB pilus formation outer membrane protein	-
HV98_RS26030	5472944..5474269	+	1326	WP_003120992.1	type 4b pilus protein PilO2	-
HV98_RS26035	5474259..5474792	+	534	WP_003120993.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5440677..5533836	93159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T47810 WP_003120987.1 NZ_CP008869:c5469905-5469606 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T47810 NZ_CP008869:c5469905-5469606 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT47810 WP_003448662.1 NZ_CP008869:c5469609-5469319 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT47810 NZ_CP008869:c5469609-5469319 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73