Detailed information of TA system TA047787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2662899..2663941
• Replicon: chromosome
• Accession: NZ_CP008866
• Organism: Pseudomonas aeruginosa strain T38079

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HW01_RS12805
• Protein ID: WP_003109777.1
• Coordinates: 2663366..2663941 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: HW01_RS12800
• Protein ID: WP_003050245.1
• Coordinates: 2662899..2663369 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW01_RS12765	2658291..2659709	-	1419	WP_003119999.1	TIGR03752 family integrating conjugative element protein	-
HW01_RS12770	2659699..2660610	-	912	WP_010791730.1	TIGR03749 family integrating conjugative element protein	-
HW01_RS12775	2660607..2661299	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
HW01_RS12780	2661296..2661694	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
HW01_RS12785	2661706..2662065	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
HW01_RS12790	2662082..2662315	-	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
HW01_RS12795	2662312..2662695	-	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
HW01_RS12800	2662899..2663369	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
HW01_RS12805	2663366..2663941	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
HW01_RS12810	2663959..2664873	+	915	WP_003120002.1	AAA family ATPase	-
HW01_RS12815	2664870..2665340	+	471	WP_003105626.1	hypothetical protein	-
HW01_RS12820	2665337..2665837	+	501	WP_003090159.1	hypothetical protein	-
HW01_RS12825	2665837..2666739	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
HW01_RS12830	2666778..2667503	+	726	WP_003120003.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2591428..2710053	118625

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T47787 WP_003109777.1 NZ_CP008866:2663366-2663941 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T47787 NZ_CP008866:2663366-2663941 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT47787 WP_003050245.1 NZ_CP008866:2662899-2663369 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT47787 NZ_CP008866:2662899-2663369 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68