Detailed information of TA system TA047783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 5601937..5602523
• Replicon: chromosome
• Accession: NZ_CP008865
• Organism: Pseudomonas aeruginosa strain S86968

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: HW02_RS26805
• Protein ID: WP_003120987.1
• Coordinates: 5602224..5602523 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: HW02_RS26800
• Protein ID: WP_003448662.1
• Coordinates: 5601937..5602227 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW02_RS26780	5597088..5597297	+	210	WP_003105733.1	cold-shock protein	-
HW02_RS26785	5597519..5599408	+	1890	WP_016851610.1	hypothetical protein	-
HW02_RS26790	5599405..5601381	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
HW02_RS26795	5601522..5601866	+	345	WP_016851612.1	hypothetical protein	-
HW02_RS26800	5601937..5602227	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
HW02_RS26805	5602224..5602523	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW02_RS26810	5602725..5603849	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
HW02_RS26815	5603849..5605558	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
HW02_RS26820	5605562..5606887	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
HW02_RS26825	5606877..5607410	+	534	WP_003099760.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5573693..5659002	85309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T47783 WP_003120987.1 NZ_CP008865:c5602523-5602224 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T47783 NZ_CP008865:c5602523-5602224 [Pseudomonas aeruginosa]
ATGGTCGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT47783 WP_003448662.1 NZ_CP008865:c5602227-5601937 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT47783 NZ_CP008865:c5602227-5601937 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
TTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCCCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCTACATTGAAAACCATGCTGGCA
GTAATGCGAGCTCTGGGAGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Structures

Toxin

• AlphaFold DB: G8CP73