Detailed information of TA system TA047778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2483570..2484612
• Replicon: chromosome
• Accession: NZ_CP008865
• Organism: Pseudomonas aeruginosa strain S86968

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HW02_RS12070
• Protein ID: WP_003109777.1
• Coordinates: 2484037..2484612 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: HW02_RS12065
• Protein ID: WP_003050245.1
• Coordinates: 2483570..2484040 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW02_RS12030	2478962..2480380	-	1419	WP_003119999.1	TIGR03752 family integrating conjugative element protein	-
HW02_RS12035	2480370..2481281	-	912	WP_010791730.1	TIGR03749 family integrating conjugative element protein	-
HW02_RS12040	2481278..2481970	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
HW02_RS12045	2481967..2482365	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
HW02_RS12050	2482377..2482736	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
HW02_RS12055	2482753..2482986	-	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
HW02_RS12060	2482983..2483366	-	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
HW02_RS12065	2483570..2484040	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
HW02_RS12070	2484037..2484612	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
HW02_RS12075	2484630..2485544	+	915	WP_003120002.1	AAA family ATPase	-
HW02_RS12080	2485541..2486011	+	471	WP_003105626.1	hypothetical protein	-
HW02_RS12085	2486008..2486508	+	501	WP_003090159.1	hypothetical protein	-
HW02_RS12090	2486508..2487410	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
HW02_RS12095	2487449..2488174	+	726	WP_003120003.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2412098..2530725	118627

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T47778 WP_003109777.1 NZ_CP008865:2484037-2484612 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T47778 NZ_CP008865:2484037-2484612 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT47778 WP_003050245.1 NZ_CP008865:2483570-2484040 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT47778 NZ_CP008865:2483570-2484040 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68