Detailed information of TA system TA047749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 934787..935829
• Replicon: chromosome
• Accession: NZ_CP008861
• Organism: Pseudomonas aeruginosa strain H47921

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HW05_RS04030
• Protein ID: WP_003153636.1
• Coordinates: 934787..935362 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: HW05_RS04035
• Protein ID: WP_003050245.1
• Coordinates: 935359..935829 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW05_RS04000	930210..931709	+	1500	WP_105264318.1	IS21 family transposase	-
HW05_RS04005	931702..932505	+	804	WP_013983152.1	IS21-like element ISPst3 family helper ATPase IstB	-
HW05_RS04010	932700..932891	-	192	Protein_811	nucleotidyltransferase	-
HW05_RS04015	932891..933391	-	501	WP_003090159.1	hypothetical protein	-
HW05_RS04020	933388..933858	-	471	WP_003090160.1	hypothetical protein	-
HW05_RS04025	933855..934769	-	915	WP_003050256.1	AAA family ATPase	-
HW05_RS04030	934787..935362	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
HW05_RS04035	935359..935829	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
HW05_RS04040	936033..936416	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
HW05_RS04045	936413..936646	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
HW05_RS04050	936663..937022	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
HW05_RS04055	937034..937432	+	399	WP_009516221.1	TIGR03750 family conjugal transfer protein	-
HW05_RS04060	937429..938121	+	693	WP_009516220.1	TIGR03746 family integrating conjugative element protein	-
HW05_RS04065	938118..939026	+	909	WP_009516219.1	TIGR03749 family integrating conjugative element protein	-
HW05_RS04070	939016..940446	+	1431	WP_009516218.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	926298..935829	9531
	-	inside	Integrative and Conjugative Element	-	-	940483..987196	46713

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T47749 WP_003153636.1 NZ_CP008861:c935362-934787 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T47749 NZ_CP008861:c935362-934787 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT47749 WP_003050245.1 NZ_CP008861:c935829-935359 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT47749 NZ_CP008861:c935829-935359 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68