Detailed information of TA system TA047747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5277665..5278305
• Replicon: chromosome
• Accession: NZ_CP008860
• Organism: Pseudomonas aeruginosa strain H27930

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HW06_RS24860
• Protein ID: WP_003105740.1
• Coordinates: 5277665..5278075 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: HW06_RS24865
• Protein ID: WP_003158175.1
• Coordinates: 5278075..5278305 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW06_RS24815	5273507..5273704	+	198	WP_003105756.1	hypothetical protein	-
HW06_RS24820	5273860..5274588	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
HW06_RS24825	5274594..5275142	+	549	WP_003105753.1	DUF3158 family protein	-
HW06_RS24830	5275189..5276028	+	840	WP_003105750.1	Rha family transcriptional regulator	-
HW06_RS24835	5276058..5276546	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
HW06_RS24840	5276654..5276899	+	246	WP_023102333.1	CrpP family protein	-
HW06_RS24845	5276965..5277183	-	219	WP_003105747.1	hypothetical protein	-
HW06_RS24855	5277383..5277643	-	261	WP_003105742.1	hypothetical protein	-
HW06_RS24860	5277665..5278075	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HW06_RS24865	5278075..5278305	-	231	WP_003158175.1	antitoxin	Antitoxin
HW06_RS24870	5278561..5280480	+	1920	WP_004352838.1	type I DNA topoisomerase	-
HW06_RS24875	5280788..5280997	+	210	WP_003105733.1	cold-shock protein	-
HW06_RS24880	5281218..5283107	+	1890	WP_059309629.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5258436..5361673	103237

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T47747 WP_003105740.1 NZ_CP008860:c5278075-5277665 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T47747 NZ_CP008860:c5278075-5277665 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT47747 WP_003158175.1 NZ_CP008860:c5278305-5278075 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT47747 NZ_CP008860:c5278305-5278075 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2