Detailed information of TA system TA047736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5334159..5334799
• Replicon: chromosome
• Accession: NZ_CP008858
• Organism: Pseudomonas aeruginosa strain F63912

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HW08_RS25745
• Protein ID: WP_003105740.1
• Coordinates: 5334159..5334569 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: HW08_RS25750
• Protein ID: WP_003158175.1
• Coordinates: 5334569..5334799 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW08_RS25700	5330001..5330198	+	198	WP_003105756.1	hypothetical protein	-
HW08_RS25705	5330354..5331082	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
HW08_RS25710	5331088..5331636	+	549	WP_003105753.1	DUF3158 family protein	-
HW08_RS25715	5331683..5332522	+	840	WP_003105750.1	Rha family transcriptional regulator	-
HW08_RS25720	5332552..5333040	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
HW08_RS25725	5333148..5333393	+	246	WP_023102333.1	CrpP family protein	-
HW08_RS25730	5333459..5333677	-	219	WP_003105747.1	hypothetical protein	-
HW08_RS25740	5333877..5334137	-	261	WP_003105742.1	hypothetical protein	-
HW08_RS25745	5334159..5334569	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HW08_RS25750	5334569..5334799	-	231	WP_003158175.1	antitoxin	Antitoxin
HW08_RS25755	5335055..5336974	+	1920	WP_004352838.1	type I DNA topoisomerase	-
HW08_RS25760	5337282..5337491	+	210	WP_003105733.1	cold-shock protein	-
HW08_RS25765	5337712..5339601	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5314589..5418165	103576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T47736 WP_003105740.1 NZ_CP008858:c5334569-5334159 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T47736 NZ_CP008858:c5334569-5334159 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTCGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGATCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATTGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGACTGGGTCGTCC
CAATCGTGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT47736 WP_003158175.1 NZ_CP008858:c5334799-5334569 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT47736 NZ_CP008858:c5334799-5334569 [Pseudomonas aeruginosa]
ATGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCAGTCGCGCTTCCCGATGACGT
TAAGCGTGTGGATGTGGTCGTTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2