Detailed information of TA system TA047734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4596739..4597997
• Replicon: chromosome
• Accession: NZ_CP008858
• Organism: Pseudomonas aeruginosa strain F63912

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: HW08_RS22005
• Protein ID: WP_003112528.1
• Coordinates: 4596739..4597494 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: HW08_RS22010
• Protein ID: WP_003086329.1
• Coordinates: 4597629..4597997 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW08_RS21995	4591911..4594454	+	2544	WP_016561630.1	acyl-homoserine-lactone acylase QuiP	-
HW08_RS22000	4594609..4595970	-	1362	WP_079276735.1	DNA recombination protein RmuC	-
HW08_RS22005	4596739..4597494	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
HW08_RS22010	4597629..4597997	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
HW08_RS22015	4598070..4599356	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
HW08_RS22020	4599412..4601001	-	1590	WP_016561628.1	aldehyde dehydrogenase family protein	-
HW08_RS22025	4601080..4601556	+	477	WP_003112531.1	hypothetical protein	-
HW08_RS32195	4601559..4601834	-	276	WP_010792073.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T47734 WP_003112528.1 NZ_CP008858:c4597494-4596739 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T47734 NZ_CP008858:c4597494-4596739 [Pseudomonas aeruginosa]
GTGAGCGAGATCTGGCGACAGTGCAAGGGTGAGCGGCATATCCGACCGCTCCAGGGGCGCCTGGTGCGGTTGGTGGAGAG
CCAGGAGCAGGTGGCGACCCTGCAATTGGTCGACACCCTGGAAGAACAGGCGTTGCTCGAGGAACTGCTCGAGTCGAGCA
AGCCGCCGGTGCCGGCGGATGCCGAGCCATTGCATTACCTGTTGAAGACCCCGTTCCGTTATCCGCCCTTGCGCTGGGGG
TCGCGGTTTGGCCGTCGCCACGAGCCAAGCCTGTTCTACGCCGCGCTGAAGCTGGAAACGGCCATGGCCGAAAGCGCCTA
TTACCGTTGCGTCCTGTGGAGTGGCATGGTGGTGCCGCCACCGAGCGGGAGGATTCTGTCCGAGCACGCTTCGTTCGAGG
CGGGCTGGAAGGTGGAGCGGGGAATCCGTCTGCAGGCGCCGCCTTTCTCCGATCACGAGGCTGCGCTGACCGACATCGCC
GACTACCGCGCTCCCCAGGAACTGGGAAGTGCCATGCGCAGCGCCGGGGTGCAGGCATTCGAATACCGTTCGGCGCGTTG
CCCGGAGCGCGGCTGCAATGTCGCCCTGTTCACGCCCGCGGCCTTCACCGAAAAGCGTCCTCGCAACCTGACGCCCTGGC
TGTGCGAAACCACGGCCGGTTACGTGGCCTTCAAGCCGGCGCATGTGCCGGGGTCGCCGAAAATCTTCTCCTGGGAGCTG
TTCCTGGTGGACGGAAAGTTGCCGCATCCGGCGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT47734 WP_003086329.1 NZ_CP008858:c4597997-4597629 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT47734 NZ_CP008858:c4597997-4597629 [Pseudomonas aeruginosa]
ATGAGCGCTCTCATCAAGGAACGTCCCAGCGCCGATGCCGTCCTGGCCAAGGCCGTCCTGGCCGCGCGCGAGCAATTGGG
GCTGACGCAGCTCGAACTGGCCGGCATCGTCGGCGTCGATCGCAGCGCCATCAGCCGCTGGAAGACCCAGGGCCTGCGGG
TGGACAGCAAGACCGGCGAGCTGGCTCTGCTGCTGGTGCGAGTCTATCGCGCACTGTATGCCCTGTTCGGCGGGCAGCAG
GAGGACATGCGCCACTTCCTGCGCACTCCCAACCATCACCTGGCGGGCGAGCCGCTGGCACTGATGGGACAGGTGCAGGG
CCTGGTTCATGTGCTGGAATACCTCGATGCGATCCGCGGCAAGGTCTGA

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7