Detailed information of TA system TA047729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 5840854..5842275
• Replicon: chromosome
• Accession: NZ_CP008857
• Organism: Pseudomonas aeruginosa strain F30658

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: HW09_RS26820
• Protein ID: WP_025297643.1
• Coordinates: 5841202..5842275 (-)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: HW09_RS34790
• Protein ID: WP_158025315.1
• Coordinates: 5840854..5841147 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW09_RS26775	5835957..5836169	+	213	WP_023128602.1	hypothetical protein	-
HW09_RS26780	5836260..5836715	-	456	WP_025297646.1	helix-turn-helix domain-containing protein	-
HW09_RS26785	5836880..5837275	+	396	WP_023128603.1	hypothetical protein	-
HW09_RS26790	5837318..5837788	+	471	WP_023128604.1	hypothetical protein	-
HW09_RS26795	5837791..5838174	+	384	WP_025297645.1	hypothetical protein	-
HW09_RS26800	5838256..5838834	+	579	WP_025297644.1	hypothetical protein	-
HW09_RS26805	5839165..5839529	+	365	Protein_5411	DNA binding protein	-
HW09_RS26810	5839753..5839947	-	195	WP_023128607.1	OprD family outer membrane porin	-
HW09_RS26815	5839922..5840715	+	794	Protein_5413	transporter substrate-binding domain-containing protein	-
HW09_RS34790	5840854..5841147	-	294	WP_158025315.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
HW09_RS26820	5841202..5842275	-	1074	WP_025297643.1	type II toxin-antitoxin system RnlA family toxin	Toxin
HW09_RS26825	5842990..5843244	+	255	WP_023128610.1	hypothetical protein	-
HW09_RS26830	5843542..5843838	+	297	WP_023128612.1	hypothetical protein	-
HW09_RS26835	5843891..5845474	-	1584	WP_025297642.1	hypothetical protein	-
HW09_RS26840	5845754..5846182	+	429	WP_025297641.1	S24 family peptidase	-
HW09_RS26845	5846169..5846414	+	246	Protein_5420	DNA polymerase V subunit UmuC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	mucE	5595540..5934965	339425

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal (6-87)

Antitoxin

• Antitoxin RnlB/LsoB (2-96)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40381.89 Da
• Isoelectric Point: 6.1830

>T47729 WP_025297643.1 NZ_CP008857:c5842275-5841202 [Pseudomonas aeruginosa]
VTERDYKNINLKRCIIEETTHCFIRDNGLKLRSYGDKPGSSGLRVVFGKAGIEDATLDIFFTNVGASTLTYKVGKNQALG
QQLADALYETIHPDEFVTMNLGIKGVDLDDAEALIKELTESANADIEVASRTSADRKHIWKLKSKKNLDELTVTQHENTH
KLQIQGRPLSCYQQLSYLLTELLEINALECILFKKEENRSEFVCAEVAESVLKERFGDCYANLPQTSRRLLLASLCVRLA
SPALPDYCMLVYPELRTLEGAIKQKLAEKNLSNQEESFGGFFTKTNGQFYLKQQYHADVGDAKLQGHLDNAYTFFNKQRH
GLFHMEEMPSVSRMISNITQAVSLCDEAYDHIKNLYS

Nucleotide

• Length: 1074 bp

>T47729 NZ_CP008857:c5842275-5841202 [Pseudomonas aeruginosa]
GTGACTGAACGCGACTACAAAAACATCAACCTCAAACGATGCATTATCGAGGAAACGACTCATTGCTTCATCCGAGATAA
TGGCCTGAAGCTGCGATCCTATGGTGACAAGCCTGGGAGTTCCGGTCTTCGAGTAGTCTTCGGAAAAGCAGGGATTGAAG
ATGCCACGCTTGATATTTTTTTCACGAATGTCGGTGCGTCTACCCTGACCTACAAGGTGGGCAAGAACCAAGCTCTCGGC
CAGCAGCTAGCGGATGCTCTTTACGAAACAATCCACCCCGATGAATTCGTAACGATGAACCTTGGTATCAAAGGCGTCGA
TCTGGATGATGCCGAAGCCCTTATCAAGGAACTCACTGAGTCAGCCAATGCGGACATTGAGGTTGCTTCGCGCACAAGCG
CTGATCGCAAGCATATTTGGAAGCTCAAGTCTAAAAAGAACTTGGATGAGTTGACTGTCACTCAGCACGAGAACACCCAT
AAACTGCAGATCCAGGGTCGTCCTCTCAGCTGCTATCAGCAGTTGAGCTACCTGCTCACCGAGCTCCTAGAGATCAACGC
ACTCGAATGCATCCTGTTCAAAAAGGAAGAAAATCGCTCGGAGTTCGTTTGTGCCGAGGTCGCGGAAAGCGTACTCAAGG
AAAGGTTTGGCGACTGCTACGCCAACCTGCCACAGACCTCGCGTAGGCTGCTCCTGGCAAGCCTTTGCGTGAGGCTTGCC
AGCCCCGCGCTACCCGACTACTGCATGCTGGTTTATCCGGAACTCCGCACCCTTGAAGGCGCGATCAAACAGAAGCTTGC
AGAAAAAAACCTTTCAAATCAGGAAGAAAGCTTCGGTGGCTTCTTTACCAAGACCAATGGTCAGTTTTATCTGAAGCAGC
AATACCATGCTGATGTCGGCGATGCTAAACTACAGGGTCATCTGGACAACGCTTACACCTTCTTCAACAAGCAACGCCAC
GGATTGTTCCACATGGAAGAGATGCCATCCGTTAGTCGAATGATTAGCAACATCACCCAAGCTGTGTCATTGTGTGATGA
AGCATATGATCATATCAAGAATTTGTATAGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11039.38 Da
• Isoelectric Point: 4.5163

>AT47729 WP_158025315.1 NZ_CP008857:c5841147-5840854 [Pseudomonas aeruginosa]
MTVVTATSYENPLSRLSIIASEMRNTSHSSKEIVLFDLLCSNGEEWNRFVSINYNGTDFEKSTCSIVSKSDIPTDLLETQ
TRFFQIHPQYLLDSVLN

Nucleotide

• Length: 294 bp

>AT47729 NZ_CP008857:c5841147-5840854 [Pseudomonas aeruginosa]
ATGACGGTAGTTACCGCTACCAGTTATGAGAACCCCTTATCTCGTCTTTCTATTATTGCTAGCGAGATGCGCAATACATC
TCACTCCAGCAAGGAGATAGTTCTATTCGATCTACTCTGCTCTAACGGAGAAGAGTGGAATCGTTTCGTCTCCATCAATT
ACAACGGCACCGACTTCGAAAAAAGCACCTGTAGCATTGTCTCCAAGTCCGACATCCCTACAGACCTCTTGGAAACACAA
ACTCGCTTCTTTCAAATCCATCCACAATATCTGCTTGATTCAGTTTTGAATTAA