Detailed information of TA system TA047723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 412700..413742
• Replicon: chromosome
• Accession: NZ_CP008857
• Organism: Pseudomonas aeruginosa strain F30658

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HW09_RS01960
• Protein ID: WP_003153636.1
• Coordinates: 413167..413742 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: HW09_RS01955
• Protein ID: WP_003050245.1
• Coordinates: 412700..413170 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW09_RS01920	408092..409510	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
HW09_RS01925	409500..410411	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
HW09_RS01930	410408..411100	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
HW09_RS01935	411097..411495	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
HW09_RS01940	411507..411866	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
HW09_RS01945	411883..412116	-	234	WP_003090170.1	TIGR03758 family integrating conjugative element protein	-
HW09_RS01950	412113..412496	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
HW09_RS01955	412700..413170	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
HW09_RS01960	413167..413742	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
HW09_RS01965	413760..414674	+	915	WP_016852809.1	AAA family ATPase	-
HW09_RS01970	414671..415141	+	471	WP_003090160.1	hypothetical protein	-
HW09_RS01975	415138..415638	+	501	WP_003090159.1	hypothetical protein	-
HW09_RS01980	415638..416540	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
HW09_RS01985	416579..417304	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	336785..459858	123073

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T47723 WP_003153636.1 NZ_CP008857:413167-413742 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T47723 NZ_CP008857:413167-413742 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAATGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT47723 WP_003050245.1 NZ_CP008857:412700-413170 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT47723 NZ_CP008857:412700-413170 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68