Detailed information of TA system TA047722

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 149045..149598
• Replicon: chromosome
• Accession: NZ_CP008857
• Organism: Pseudomonas aeruginosa strain F30658

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: HW09_RS33660
• Protein ID: WP_078463403.1
• Coordinates: 149305..149598 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A7U7I516
• Locus tag: HW09_RS00785
• Protein ID: WP_023127699.1
• Coordinates: 149045..149317 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW09_RS00765	144443..146005	+	1563	WP_059319057.1	hypothetical protein	-
HW09_RS00770	146028..146663	+	636	WP_059319058.1	hypothetical protein	-
HW09_RS00775	146676..147698	+	1023	WP_059319059.1	hypothetical protein	-
HW09_RS33650	147752..148048	+	297	WP_078463402.1	YkgJ family cysteine cluster protein	-
HW09_RS00780	148699..148887	+	189	Protein_161	IS5/IS1182 family transposase	-
HW09_RS00785	149045..149317	+	273	WP_023127699.1	CopG family ribbon-helix-helix protein	Antitoxin
HW09_RS33660	149305..149598	+	294	WP_078463403.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HW09_RS00790	149592..151001	-	1410	WP_023127700.1	site-specific integrase	-
HW09_RS00800	151860..153785	+	1926	WP_015649025.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11154.67 Da
• Isoelectric Point: 7.9865

>T47722 WP_078463403.1 NZ_CP008857:149305-149598 [Pseudomonas aeruginosa]
VPRLIVTEGAAKGLERCRRFLSDKDPQVARRAAQAIERQFARLEKSPEVGRPFPDLPELRELIIEFGDSGYVALYRYERA
DDTAYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T47722 NZ_CP008857:149305-149598 [Pseudomonas aeruginosa]
GTGCCACGATTGATTGTTACTGAAGGCGCGGCGAAGGGCTTGGAGCGTTGCCGGCGATTCCTCTCAGATAAAGATCCGCA
GGTCGCGCGCCGTGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGAAAAGTCCGGAGGTAGGGCGTCCATTTC
CAGATCTGCCGGAGCTGCGTGAGCTCATCATCGAGTTTGGAGACTCAGGTTACGTAGCGTTGTACCGCTATGAGCGTGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10493.62 Da
• Isoelectric Point: 5.6460

>AT47722 WP_023127699.1 NZ_CP008857:149045-149317 [Pseudomonas aeruginosa]
MATSIKIDDELKGRIQQLASQRQRSSHWIMREAITQYVEREEARESFKQEALASWAAYQETGRHLTSQEARTWLGSWGTD
TEVELPKCHD

Nucleotide

• Length: 273 bp

>AT47722 NZ_CP008857:149045-149317 [Pseudomonas aeruginosa]
ATGGCCACTTCAATCAAAATTGATGACGAGCTAAAAGGTCGCATTCAGCAGTTGGCGAGCCAACGCCAGCGCTCGTCTCA
CTGGATCATGCGCGAGGCGATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTCAAGCAGGAAGCCCTCGCAT
CGTGGGCGGCGTATCAGGAAACCGGAAGGCATTTGACCAGCCAAGAAGCACGGACCTGGCTCGGTAGCTGGGGTACAGAT
ACTGAGGTAGAGCTTCCCAAGTGCCACGATTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U7I516