Detailed information of TA system TA047721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 140441..141075
• Replicon: chromosome
• Accession: NZ_CP008857
• Organism: Pseudomonas aeruginosa strain F30658

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: L1LRL5
• Locus tag: HW09_RS00750
• Protein ID: WP_009408500.1
• Coordinates: 140671..141075 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L1LS63
• Locus tag: HW09_RS00745
• Protein ID: WP_003285520.1
• Coordinates: 140441..140671 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HW09_RS00720	136489..137475	+	987	WP_059319051.1	hypothetical protein	-
HW09_RS00725	137576..137758	-	183	WP_059319052.1	hypothetical protein	-
HW09_RS00730	137769..138266	-	498	WP_059319053.1	DNA repair protein RadC	-
HW09_RS00735	138238..139188	-	951	WP_059319054.1	hypothetical protein	-
HW09_RS00740	139280..140284	-	1005	WP_059319055.1	YqaJ viral recombinase family protein	-
HW09_RS00745	140441..140671	+	231	WP_003285520.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HW09_RS00750	140671..141075	+	405	WP_009408500.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HW09_RS00755	141162..142130	-	969	WP_059319056.1	DUF932 domain-containing protein	-
HW09_RS35130	142650..143029	-	380	Protein_155	DUF4113 domain-containing protein	-
HW09_RS00760	143403..144446	+	1044	WP_017940252.1	WYL domain-containing protein	-
HW09_RS00765	144443..146005	+	1563	WP_059319057.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14977.20 Da
• Isoelectric Point: 7.2774

>T47721 WP_009408500.1 NZ_CP008857:140671-141075 [Pseudomonas aeruginosa]
MLKYMLDTNICIFTIKNRPEQVREAFKRHSGQLSISTVTLMELIYGAEKSANPERNLADVEGFAARLEVLPYDAQAAAHS
GQLRAELARIGKPIGPYDQMIAGHARAQGLILVTNNLREFERVPGLRVEDWVNA

Nucleotide

• Length: 405 bp

>T47721 NZ_CP008857:140671-141075 [Pseudomonas aeruginosa]
ATGCTGAAGTACATGCTTGATACCAACATCTGCATTTTCACGATCAAGAATCGCCCTGAGCAGGTGCGGGAAGCCTTCAA
GCGCCATTCAGGCCAGCTAAGCATCAGCACTGTCACCCTGATGGAGCTTATTTACGGCGCCGAGAAGTCCGCTAATCCCG
AGCGTAATCTGGCGGACGTTGAAGGTTTCGCTGCGCGCCTGGAAGTACTGCCTTACGACGCTCAGGCAGCGGCTCATTCC
GGGCAATTGCGAGCTGAGCTGGCTCGTATCGGCAAGCCTATTGGCCCGTATGATCAGATGATTGCAGGACACGCACGTGC
CCAGGGACTGATTCTGGTCACGAATAATCTTCGTGAGTTTGAGCGAGTTCCAGGTTTGCGCGTCGAGGATTGGGTGAACG
CCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8432.41 Da
• Isoelectric Point: 4.2683

>AT47721 WP_003285520.1 NZ_CP008857:140441-140671 [Pseudomonas aeruginosa]
MEQGAVFQSNRSQAVRLPKAVALPEDVKRVDVVVVGRARIITPAGEAWDVWFDGEGVTADYMADREQPADQERDAF

Nucleotide

• Length: 231 bp

>AT47721 NZ_CP008857:140441-140671 [Pseudomonas aeruginosa]
ATGGAACAAGGTGCAGTTTTTCAGAGCAACCGCAGTCAGGCGGTGCGTTTGCCCAAGGCGGTTGCGCTGCCTGAGGATGT
GAAGCGGGTGGATGTAGTGGTAGTTGGTCGAGCCCGAATCATCACGCCGGCAGGCGAGGCCTGGGACGTCTGGTTCGATG
GCGAAGGTGTGACCGCTGACTACATGGCGGATCGCGAGCAACCTGCAGATCAAGAGCGGGATGCGTTCTGA

Structures

Toxin

• AlphaFold DB: L1LRL5

Antitoxin

• AlphaFold DB: L1LS63