Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 110515..111041 Replicon plasmid pKOXM1B
Accession NZ_CP008843
Organism Klebsiella michiganensis strain M1

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag HR38_RS28725 Protein ID WP_000323025.1
Coordinates 110754..111041 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID J5W3H0
Locus tag HR38_RS28720 Protein ID WP_004196370.1
Coordinates 110515..110754 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR38_RS31960 105852..106044 - 193 Protein_119 MFS transporter -
HR38_RS28695 106074..107051 + 978 WP_004196334.1 chromate resistance protein -
HR38_RS28700 107008..108384 + 1377 WP_004196363.1 chromate efflux transporter -
HR38_RS28705 108415..109104 - 690 WP_004196322.1 hypothetical protein -
HR38_RS28710 109118..109855 - 738 WP_008460272.1 HupE/UreJ family protein -
HR38_RS30950 109899..110264 - 366 WP_009651956.1 hypothetical protein -
HR38_RS28720 110515..110754 + 240 WP_004196370.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
HR38_RS28725 110754..111041 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
HR38_RS28730 111209..112708 + 1500 WP_004118622.1 IS21 family transposase -
HR38_RS28735 112705..113460 + 756 WP_038423655.1 IS21-like element ISEc10 family helper ATPase IstB -
HR38_RS28740 113509..114558 - 1050 WP_040118510.1 IS481-like element ISKpn28 family transposase -
HR38_RS28745 114630..114788 + 159 WP_014386184.1 type I toxin-antitoxin system Hok family toxin -
HR38_RS28750 115228..115500 + 273 WP_032426567.1 hypothetical protein -
HR38_RS28755 115497..115847 + 351 WP_004152758.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..183015 183015


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(3-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T47705 WP_000323025.1 NZ_CP008843:110754-111041 [Klebsiella michiganensis]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T47705 NZ_CP008843:110754-111041 [Klebsiella michiganensis]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9018.43 Da        Isoelectric Point: 4.3894

>AT47705 WP_004196370.1 NZ_CP008843:110515-110754 [Klebsiella michiganensis]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLCNPKPVRVTLDEL

Download         Length: 240 bp

>AT47705 NZ_CP008843:110515-110754 [Klebsiella michiganensis]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTTGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4QRH4

References