Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 160973..161709 Replicon plasmid pKOXM1A
Accession NZ_CP008842
Organism Klebsiella michiganensis strain M1

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HR38_RS27905 Protein ID WP_003026803.1
Coordinates 161227..161709 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HR38_RS27900 Protein ID WP_003026799.1
Coordinates 160973..161239 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR38_RS27880 157167..158456 + 1290 WP_000922630.1 arsenite efflux transporter membrane subunit ArsB -
HR38_RS27885 158469..158894 + 426 WP_000065758.1 glutaredoxin-dependent arsenate reductase -
HR38_RS27890 158925..159191 - 267 WP_102047566.1 hypothetical protein -
HR38_RS27895 159265..159969 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
HR38_RS30835 160023..160703 - 681 Protein_171 hypothetical protein -
HR38_RS27900 160973..161239 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HR38_RS27905 161227..161709 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HR38_RS30840 161909..162826 + 918 Protein_174 ISNCY family transposase -
HR38_RS27915 162916..164415 + 1500 WP_004118622.1 IS21 family transposase -
HR38_RS27920 164412..165167 + 756 WP_038423655.1 IS21-like element ISEc10 family helper ATPase IstB -
HR38_RS31900 165234..165683 + 450 Protein_177 ISNCY family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..204468 204468
- inside IScluster/Tn - - 159265..168032 8767


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T47704 WP_003026803.1 NZ_CP008842:161227-161709 [Klebsiella michiganensis]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T47704 NZ_CP008842:161227-161709 [Klebsiella michiganensis]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT47704 WP_003026799.1 NZ_CP008842:160973-161239 [Klebsiella michiganensis]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT47704 NZ_CP008842:160973-161239 [Klebsiella michiganensis]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References