Detailed information of TA system TA047702

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 95147..95682
• Replicon: plasmid pKOXM1A
• Accession: NZ_CP008842
• Organism: Klebsiella michiganensis strain M1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A6M4NRI2
• Locus tag: HR38_RS27570
• Protein ID: WP_032693963.1
• Coordinates: 95395..95682 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8G2A2B7
• Locus tag: HR38_RS27565
• Protein ID: WP_032752108.1
• Coordinates: 95147..95398 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR38_RS27535	90516..90795	-	280	Protein_98	hypothetical protein	-
HR38_RS30760	91113..91343	-	231	WP_047665741.1	hypothetical protein	-
HR38_RS27545	91407..92078	-	672	WP_032700854.1	plasmid partitioning/stability family protein	-
HR38_RS27550	92081..93052	-	972	WP_077255421.1	StbA family protein	-
HR38_RS27555	93271..93714	+	444	WP_040118420.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
HR38_RS27560	93714..94982	+	1269	WP_032752101.1	Y-family DNA polymerase	-
HR38_RS27565	95147..95398	+	252	WP_032752108.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HR38_RS27570	95395..95682	+	288	WP_032693963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HR38_RS27575	96775..97124	+	350	Protein_106	DUF4113 domain-containing protein	-
HR38_RS27580	97848..98819	-	972	WP_032752114.1	ParB/RepB/Spo0J family plasmid partition protein	-
HR38_RS27585	98819..99985	-	1167	WP_032752115.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..204468	204468

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11133.12 Da
• Isoelectric Point: 10.2246

>T47702 WP_032693963.1 NZ_CP008842:95395-95682 [Klebsiella michiganensis]
MTYTVKFREDALKEWQKLDKAIQQQFAKKLKKCCDEPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDDQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T47702 NZ_CP008842:95395-95682 [Klebsiella michiganensis]
ATGACTTATACGGTCAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAGCTGGATAAGGCCATTCAGCAGCAGTTTGC
GAAGAAACTGAAGAAGTGCTGTGACGAACCACATATCCCTTCAGCAAAGCTGAGAGGAATAAAGGACTGCTACAAAATTA
AACTTCGTGCATCAGGTTTTCGCCTGGTGTATCAGGTGATAGATGATCAATTAATTATCGCGGTTGTTGCTGTTGGAAAG
CGTGAACGCAGTGACGTGTACAATCTGGCCAGCGAAAGGATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9174.43 Da
• Isoelectric Point: 3.9507

>AT47702 WP_032752108.1 NZ_CP008842:95147-95398 [Klebsiella michiganensis]
MPNIILSDTSASISELKKNPMATVSAGDGFPVAILNRNQPAFYCIPAELYEKMLDALDDQELAKLVSERSNQQLLDVDLD
TYL

Nucleotide

• Length: 252 bp

>AT47702 NZ_CP008842:95147-95398 [Klebsiella michiganensis]
ATGCCCAACATTATTCTGAGTGACACAAGCGCGAGCATCAGCGAACTAAAAAAAAATCCGATGGCGACGGTCAGTGCCGG
CGACGGCTTCCCGGTCGCGATTCTGAACCGTAACCAGCCGGCTTTTTACTGCATCCCGGCTGAACTTTACGAGAAGATGC
TCGATGCCCTGGATGATCAGGAACTGGCAAAGCTGGTATCAGAACGCAGTAACCAGCAGCTGCTTGACGTGGATCTGGAT
ACTTATCTATGA

Structures

Toxin

• AlphaFold DB: A0A6M4NRI2