Detailed information of TA system TA047692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 1782637..1783213
• Replicon: chromosome
• Accession: NZ_CP008841
• Organism: Klebsiella michiganensis strain M1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HR38_RS08365
• Protein ID: WP_038424021.1
• Coordinates: 1782637..1782924 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A0H3H591
• Locus tag: HR38_RS08370
• Protein ID: WP_014227757.1
• Coordinates: 1782911..1783213 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR38_RS08335	1777979..1778497	-	519	WP_004098240.1	hypothetical protein	-
HR38_RS08340	1778494..1779294	-	801	WP_014837308.1	winged helix-turn-helix domain-containing protein	-
HR38_RS08345	1779440..1780024	-	585	WP_004098242.1	TetR/AcrR family transcriptional regulator	-
HR38_RS08350	1780172..1780954	-	783	WP_014837310.1	NAD(P)H-dependent oxidoreductase	-
HR38_RS08355	1781042..1781950	+	909	WP_014837311.1	LysR family transcriptional regulator	-
HR38_RS08360	1781944..1782387	+	444	WP_014837312.1	FosA family fosfomycin resistance glutathione transferase	-
HR38_RS08365	1782637..1782924	+	288	WP_038424021.1	BrnT family toxin	Toxin
HR38_RS08370	1782911..1783213	+	303	WP_014227757.1	BrnA antitoxin family protein	Antitoxin
HR38_RS08375	1783334..1784677	-	1344	WP_032719935.1	APC family permease	-
HR38_RS08380	1784674..1785585	-	912	WP_032719936.1	acetamidase/formamidase family protein	-
HR38_RS08385	1786038..1786373	-	336	WP_014227760.1	endoribonuclease SymE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (38-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11253.72 Da
• Isoelectric Point: 8.5899

>T47692 WP_038424021.1 NZ_CP008841:1782637-1782924 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTLGLVHGIIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T47692 NZ_CP008841:1782637-1782924 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCGAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAGGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11607.18 Da
• Isoelectric Point: 9.4429

>AT47692 WP_014227757.1 NZ_CP008841:1782911-1783213 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Nucleotide

• Length: 303 bp

>AT47692 NZ_CP008841:1782911-1783213 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGATATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3H591